Product nameAnti-V5 tag antibody [E10]
See all V5 tag primary antibodies
DescriptionMouse monoclonal [E10] to V5 tag
Specificityab53418 detects (GKPIPNPLLGLDST) V5 tag fusion protein.
Tested applicationsSuitable for: ICC/IF, IHC-P, WB, ELISA, IHC-Frmore details
Species reactivityHuman, mouse, or other cell types having the GKPIPNPLLGLDST tag.
- 293 cells untransfected and transfected with lacZ-V5-tagged.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 0.0268% PBS
Concentration information loading...
PurityProtein A purified
Purification notesab53418 was affinity purified from culture supernatant of hybridoma cells grown in a bioreactor.
Our Abpromise guarantee covers the use of ab53418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/2000.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/1000 - 1/3000. Detects a band of approximately 130 kDa.|
|ELISA||1/5000 - 1/10000.|
|IHC-Fr||1/500 - 1/2000.|
RelevanceThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
ab53418 staining V5 tag in Mouse B16F1 melanoma cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Tween 20 and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in 1% BSA + 0.5% Tween 20 + 1% Donkey serum) for 1 hour at 21°C. An Alexa Fluor® 555-conjugated Donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
Melonma cells expressing GFP are also expressing GFP (AF55) due to transfected cell line with GFP-V5.
All lanes : Anti-V5 tag antibody [E10] (ab53418) at 1/1000 dilution
Lane 1 : 293 cells
Lane 2 : 293 cells
Lysates/proteins at 30 µg per lane.
Observed band size: 130 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Amabile A et al. Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing. Cell 167:219-232.e14 (2016). WB . Read more (PubMed: 27662090) »
- Wang J et al. Sumoylation modulates 20-hydroxyecdysone signaling by maintaining USP protein levels in Drosophila. Insect Biochem Mol Biol 54C:80-88 (2014). Read more (PubMed: 25240618) »