Anti-V5 tag antibody [E10] (ab53418)
![Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody [E10] (ab53418)](https://www.abcam.com/ps/products/53/ab53418/Images/ab53418-212030-anti-v5-tag-antibody-e10-immunofluorescence.jpg "Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody [E10] (ab53418)")
Key features and details
- Mouse monoclonal [E10] to V5 tag
- Suitable for: ICC/IF, IHC-P, WB, ELISA, IHC-Fr
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-V5 tag antibody [E10]
See all V5 tag primary antibodies -
Description
Mouse monoclonal [E10] to V5 tag -
Host species
Mouse -
Specificity
ab53418 detects (GKPIPNPLLGLDST) V5 tag fusion protein. -
Tested applications
Suitable for: ICC/IF, IHC-P, WB, ELISA, IHC-Frmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
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Positive control
- 293 cells untransfected and transfected with lacZ-V5-tagged.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
ab53418 was affinity purified from culture supernatant of hybridoma cells grown in a bioreactor. -
Clonality
Monoclonal -
Clone number
E10 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53418 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
1/500 - 1/2000.
|
| IHC-P |
Use at an assay dependent concentration.
|
|
| WB | (5) |
1/1000 - 1/3000. Detects a band of approximately 130 kDa.
|
| ELISA |
1/5000 - 1/10000.
|
|
| IHC-Fr |
1/500 - 1/2000.
|
| Notes |
|---|
|
ICC/IF
1/500 - 1/2000. |
|
IHC-P
Use at an assay dependent concentration. |
|
WB
1/1000 - 1/3000. Detects a band of approximately 130 kDa. |
|
ELISA
1/5000 - 1/10000. |
|
IHC-Fr
1/500 - 1/2000. |
Target
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Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody [E10] (ab53418)This image is courtesy of an Abreview submitted by Javier Rodriguez
ab53418 staining V5 tag in Mouse B16F1 melanoma cell line by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.5% Tween 20 and blocked with 3% BSA for 1 hour at 23°C. Samples were incubated with primary antibody (1/500 in 1% BSA + 0.5% Tween 20 + 1% Donkey serum) for 1 hour at 21°C. An Alexa Fluor® 555-conjugated Donkey anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody.
Melonma cells expressing GFP are also expressing GFP (AF55) due to transfected cell line with GFP-V5.
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![Western blot - Anti-V5 tag antibody [E10] (ab53418)](https://www.abcam.com/ps/products/53/ab53418/Images/ab53418-66175-anti-v5-tag-antibody-e10-western-blot.gif)
Western blot - Anti-V5 tag antibody [E10] (ab53418)All lanes : Anti-V5 tag antibody [E10] (ab53418) at 1/1000 dilution
Lane 1 : 293 cells
untransfected (control)
Lane 2 : 293 cells
transfected
with lacZ-V5-tagged.
Lysates/proteins at 30 µg per lane.
Observed band size: 130 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab53418 has been referenced in 6 publications.
- Mora Gallardo C et al. Dido3-dependent SFPQ recruitment maintains efficiency in mammalian alternative splicing. Nucleic Acids Res 47:5381-5394 (2019). PubMed: 30931476
- Aoki SK et al. A universal biomolecular integral feedback controller for robust perfect adaptation. Nature 570:533-537 (2019). PubMed: 31217585
- Amabile A et al. Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing. Cell 167:219-232.e14 (2016). WB . PubMed: 27662090
- Wang J et al. Sumoylation modulates 20-hydroxyecdysone signaling by maintaining USP protein levels in Drosophila. Insect Biochem Mol Biol 54C:80-88 (2014). PubMed: 25240618
- Cho SW et al. Novel role of hnRNP-A2/B1 in modulating aryl hydrocarbon receptor ligand sensitivity. Arch Toxicol N/A:N/A (2014). PubMed: 25224401
- Zhang F et al. The African swine fever virus DP71L protein recruits the protein phosphatase 1 catalytic subunit to dephosphorylate eIF2alpha and inhibits CHOP induction but is dispensable for these activities during virus infection. J Virol 84:10681-9 (2010). PubMed: 20702639
