HRP Anti-V5 tag antibody [E10/V4RR] (ab173837)
Key features and details
- HRP Mouse monoclonal [E10/V4RR] to V5 tag
- Suitable for: WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG1
Overview
-
Product name
HRP Anti-V5 tag antibody [E10/V4RR]
See all V5 tag primary antibodies -
Description
HRP Mouse monoclonal [E10/V4RR] to V5 tag -
Host species
Mouse -
Conjugation
HRP -
Specificity
ab173837 shows reactivity with tagged proteins.
-
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to V5 tag conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:GKPIPNPLLGLDST
-
Positive control
- E. coli lysate containing a V5 multi-epitope tagged protein.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Concentration information loading...
-
Clonality
Monoclonal -
Clone number
E10/V4RR -
Isotype
IgG1 -
Research areas
Associated products
-
Compatible Secondaries
-
Related Products
- V5 tag peptide (ab15829)
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
- 450 nm Stop Solution for TMB Substrate (ab171529)
- 650 nm Stop Solution for TMB Substrate (ab171531)
- Immunoassay Blocking Buffer (ab171534)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab173837 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/2000.
|
Notes |
---|
WB
1/500 - 1/2000. |
Target
-
Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Images
-
All lanes : HRP Anti-V5 tag antibody [E10/V4RR] (ab173837) at 1/1000 dilution
Lane 1 : E. coli lysate containing a V5 multi-epitope tagged protein at 1 µg
Lane 2 : E. coli lysate containing a V5 multi-epitope tagged protein at 0.5 µg
Lane 3 : E. coli lysate containing a V5 multi-epitope tagged protein at 0.25 µg
Lane 4 : E. coli lysate containing a V5 multi-epitope tagged protein at 0.125 µg
Lane 5 : E. coli lysate containing a V5 multi-epitope tagged protein at 0.0599999986588955 µg
Lane 6 : E. coli lysate containing a V5 multi-epitope tagged protein at 0.0299999993294477 µg
Developed using the ECL technique.
Datasheets and documents
-
Datasheet download
References (0)
ab173837 has not yet been referenced specifically in any publications.