RecombinantRabMAb

Recombinant Anti-V5 tag antibody [EPR12989] (ab182008)

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Western blot - Anti-V5 tag antibody [EPR12989] (ab182008)
  • Anti-V5 tag antibody [EPR12989] (ab182008)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12989] to V5 tag
  • Suitable for: WB
  • Reacts with: Species independent

Overview

  • Product name

    Anti-V5 tag antibody [EPR12989]
    See all V5 tag primary antibodies

  • Description

    Rabbit monoclonal [EPR12989] to V5 tag

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Species independent

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Multi-tag fusion protein.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR12989

  • Isotype

    IgG

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab182008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/50000. Predicted molecular weight: 32 kDa.
Notes
WB
1/1000 - 1/50000. Predicted molecular weight: 32 kDa.

Target

  • Relevance

    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

  • Alternative names

    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Images

  • Western blot - Anti-V5 tag antibody [EPR12989] (ab182008)
    Western blot - Anti-V5 tag antibody [EPR12989] (ab182008)
    Anti-V5 tag antibody [EPR12989] (ab182008) at 1/50000 dilution + multi-tag fusion protein, 5ng

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 32 kDa



    Blocking/ Dilution buffer: 5% NFDM /TBST.

  • Anti-V5 tag antibody [EPR12989] (ab182008)
    Anti-V5 tag antibody [EPR12989] (ab182008)

References (0)

Publishing research using ab182008? Please let us know so that we can cite the reference in this datasheet.

ab182008 has not yet been referenced specifically in any publications.

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