Recombinant Anti-V5 tag antibody [EPR12989] (ab182008)
![Western blot - Anti-V5 tag antibody [EPR12989] (ab182008)](https://www.abcam.com/ps/products/182/ab182008/Images/ab182008-215346-anti-v5-tag-antibody-epr12989-western-blot.jpg "Western blot - Anti-V5 tag antibody [EPR12989] (ab182008)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12989] to V5 tag
- Suitable for: WB
- Reacts with: Species independent
Related conjugates and formulations
Overview
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Product name
Anti-V5 tag antibody [EPR12989]
See all V5 tag primary antibodies -
Description
Rabbit monoclonal [EPR12989] to V5 tag -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Multi-tag fusion protein.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12989 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab182008 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/50000. Predicted molecular weight: 32 kDa.
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| Notes |
|---|
|
WB
1/1000 - 1/50000. Predicted molecular weight: 32 kDa. |
Target
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Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Images
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Western blot - Anti-V5 tag antibody [EPR12989] (ab182008)Anti-V5 tag antibody [EPR12989] (ab182008) at 1/50000 dilution + multi-tag fusion protein, 5ng
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 32 kDaBlocking/ Dilution buffer: 5% NFDM /TBST.
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![Anti-V5 tag antibody [EPR12989] (ab182008)](https://www.abcam.com/ps/products/182/ab182008/Images/ab182008-2-benefits-of-recombinant-antibodies.png)
Anti-V5 tag antibody [EPR12989] (ab182008)
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (1)
ab182008 has been referenced in 1 publication.
- Balogh E et al. Pseudouridylation defect due to DKC1 and NOP10 mutations causes nephrotic syndrome with cataracts, hearing impairment, and enterocolitis. Proc Natl Acad Sci U S A 117:15137-15147 (2020). PubMed: 32554502
