• Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Sodium azide
    Constituent: 0.2% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab1274 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC 1/200 - 1/2000.
ICC/IF Use at an assay dependent concentration. PubMed: 18285447


  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all


  • ab1274 staining V5 tag (FITC) in transfected COS-7 cells by Immunocytochemistry/ Immunofluorescence.
    Drosophila thrombospondins were expressed with V5-epitope tags and detected with ab1274. Thrombospondins are retained in the extracellular matrix of the COS-7 cells. Scale bar: 20 µm.


This product has been referenced in:
  • Francis O  et al. Molecular Phylogeny of a RING E3 Ubiquitin Ligase, Conserved in Eukaryotic Cells and Dominated by Homologous Components, the Muskelin/RanBPM/CTLH Complex. PLoS One 8:e75217 (2013). ICC/IF . Read more (PubMed: 24143168) »
  • Linka Y  et al. The impact of TEL-AML1 (ETV6-RUNX1) expression in precursor B cells and implications for leukaemia using three different genome-wide screening methods. Blood Cancer J 3:e151 (2013). Read more (PubMed: 24121163) »

See all 4 Publications for this product

Customer reviews and Q&As

DISCOUNT CODE: XXXXXX Expiration date: 20-Apr-2012 I am very pleased to hear you would like to accept our offer and test ab9116 in IHC-FR (4% PFA fixed Drosophila sections). This code will give you 1 free [PRIMARY ANTIBODY] before the expiration d...

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Thank you for contacting us. ab1274, ab9116 and ab98795 are not covered under Abpromise guarantee as per customer request. I am including other product under IHC-Fr application with PFA as fixative. Ab98795 is a secondary antibody; Abreview discount co...

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Thank you for contacting us. These antibodies are against tags so species is not applicable for these products. "10 min in 4% paraformaldehyde and proceeds with the staining" is not enough information that we need for any recommendations. Pleas...

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Thank you for your patience. I have heard back from the originator of this antibody and your protocol looks fine, especially since the monoclonal worked under the same conditions. There may have been a problem with the vial/lot that you received and I ...

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Thank you for returning the vial of ab1274 to us which we received today. There was powder and no liquid in the vial which is extremely bizarre; all the other vials of that same lot are fine. A new vial of ab1274 will be shipped out to your attention t...

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What is the lot number (it is on the vial) that you received? I have checked some of the vials of ab1274 that we have in stock and they are all in liquid form. Did you spin the vial down?

Thank you for your enquiry. Ab1274 has so far only been tested for application in immunocytochemistry and immunohistochemistry. We don't have have any data to this point as to how ab1274 will work in immunofluorescence. If you decide to go ahead and...

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