Overview

  • Product name
    Anti-V5 tag antibody (HRP)
    See all V5 tag primary antibodies
  • Description
    Rabbit polyclonal to V5 tag (HRP)
  • Host species
    Rabbit
  • Conjugation
    HRP
  • Specificity
    In a direct ELISA against 1 ug of V5 peptide coupled to BSA, conjugate diluted to 1/20,000 produced a 1.0 OD in a 15 minute reaction with TMB as substrate.
  • Tested applications
    Suitable for: ICC, WB, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Synthetic peptide: GKPIPNPLLGLDST conjugated to KLH.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: PBS, 0.2% BSA
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Antibodies were immunoaffinity purified using the peptide conjugated to a solid phase support and conjugated to horseradish peroxidase.
  • Clonality
    Polyclonal
  • Isotype
    unknown
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1325 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 1 kDa.Can be blocked with V5 tag peptide (ab15829).
ELISA Use at an assay dependent dilution.

Target

  • Relevance
    The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
  • Alternative names
    • GKPIPNPLLGLDST epitope tag antibody
    • GKPIPNPLLGLDST tag antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • rev antibody
    • V5 epitope tag antibody
    see all

Images

  • All lanes : Anti-V5 tag antibody (HRP) (ab1325) at 0.2 µg/ml

    Lane 1 : E. coli whole cell lysate at 0.2 µg
    Lane 2 : E. coli whole cell lysate at 0.1 µg
    Lane 3 : E. coli whole cell lysate at 0.05 µg

    Predicted band size: 1 kDa

References

This product has been referenced in:
  • Xu J  et al. Nuclear carbonic anhydrase 6B associates with PRMT5 to epigenetically promote IL-12 expression in innate response. Proc Natl Acad Sci U S A 114:8620-8625 (2017). Read more (PubMed: 28739930) »
  • Gin P  et al. Chylomicronemia mutations yield new insights into interactions between lipoprotein lipase and GPIHBP1. Hum Mol Genet 21:2961-72 (2012). ELISA . Read more (PubMed: 22493000) »
See all 3 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Western blot
Loading amount
0.1 µg
Gel Running Conditions
Reduced Denaturing (4~20%)
Sample
Escherichia coli Recombinant protein (Recombinant protein with C-terminus V5 tag)
Specification
Recombinant protein with C-terminus V5 tag
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted May 30 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Recombinant protein (Recombinant V5 tagged Protein)
Specification
Recombinant V5 tagged Protein
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Type
Direct

Abcam user community

Verified customer

Submitted Aug 11 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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