Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [SV5-P-K] to V5 tag
- Suitable for: ELISA, ICC/IF, IHC-Fr, IP, RIA, WB
- Reacts with: Species independent
Product nameAnti-V5 tag antibody [SV5-P-K]
See all V5 tag primary antibodies
DescriptionHuman monoclonal [SV5-P-K] to V5 tag
Specificityab206560 recognises a 14 amino acid sequence (GKPIPNPLLGLDST) derived from the P and V proteins of the paramyxovirus simian virus 5.
Tested applicationsSuitable for: ELISA, ICC/IF, IHC-Fr, IP, RIA, WBmore details
Species reactivityReacts with: Species independent
ab206560 is a recombinant monoclonal antibody to V5 epitope tag, manufactured using variable regions (i.e. specificity) from the hybridoma SV5-P-K. This is a chimeric human Fab fragment with a his-tag, based on the original mouse IgG1 format, created for improved compatibility with existing reagents, assays and techniques.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Proclin 300
Constituent: 99% PBS
Concentration information loading...
Purification notesPurified by Immobilized Metal Affinity Chromatography.
Light chain typekappa
- Anti-V5 tag antibody [SV5-P-K] (ab206557)
- Anti-V5 tag antibody [SV5-P-K] (ab206558)
- Anti-V5 tag antibody [SV5-P-K] (ab206559)
- Anti-V5 tag antibody [SV5-P-K] (ab206561)
- Anti-V5 tag antibody [SV5-P-K] (ab206562)
- Anti-V5 tag antibody [SV5-P-K] (ab206565)
- Anti-V5 tag antibody [SV5-P-K] (ab206566)
- Anti-V5 tag antibody [SV5-P-K] (ab206571)
Our Abpromise guarantee covers the use of ab206560 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|RIA||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration.|
RelevanceThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab206560 has not yet been referenced specifically in any publications.