Product nameAnti-V5 tag antibody [SV5-Pk1]
See all V5 tag primary antibodies
DescriptionMouse monoclonal [SV5-Pk1] to V5 tag
Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Fr, ELISA, IP, WB, RIAmore details
Species reactivityReacts with: Species independent
Mice were infected with the paramyxovirus SV5, Simian-Virus 5.
Epitopeab27671 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
- WB: Transfected NIH/3T3 and COS-7 cell lysates. ICC/IF: CHO cells overexpressing a V5-tagged protein.
ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Concentration information loading...
PurityProtein A purified
Purification notesThis antibody was purified from tissue culture supernatant by affinity chromatography.
Primary antibody notesAb27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
Our Abpromise guarantee covers the use of ab27671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21887337|
|Flow Cyt||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||1/1000 - 1/5000.|
|IP||Use at an assay dependent concentration.|
|WB||1/1000 - 1/5000.|
|RIA||Use at an assay dependent concentration.|
RelevanceThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
ab27671 at a 1/1000 dilution staining V5 tag in CHO (Chinese hamster ovary cell line) cells overexpressing a V5-tagged protein by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton X-100. Samples were blocked with 3% BSA. The secondary used was an Alexa Fluor® 488 conjugated goat anti-mouse IgG (H+L) at a 1/1000 dilution.
All lanes : Anti-V5 tag antibody [SV5-Pk1] (ab27671) at 1/1000 dilution
Lanes 1-2 : Transfected NIH/3T3 cell lysate
Lane 3 : Not transfected NIH/3T3 cell lysate
All lanes : HRP-conjugated rabbit polyclonal Anti-mouse
Developed using the ECL technique.
Exposure time: 1 second
Cells were transfected with a V5-tagged HSPB8 (MW:~25kD) plasmid, which expressed a V5-tagged protein with molecular weight of approximately 28kD.
γSMA is expressed in TGF-β-induced EMT.
HuH7 (Human cell line) cells were transfected with a V5-tagged vector coding for γSMA. Cells were treated with TGF-β for 48h, and the expression of V5, γSMA and αSMA was determined by immunofluorescence analysis. Expression levels of V5 and γSMA or V5 and αSMA were determined by measuring fluorescence intensity on a linear section of the cell.
One representative experiment is shown.
Characterization of new C9orf72 dipeptide repeat antibodies.
Rabbit polyclonal antibodies were generated against the sense strand-derived DPRs GA and GR.
(A) Western blot of lysates from COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with the indicated vectors. Blots were serially probed with the indicated DPR antibody, then ab27671 and then actin as a loading control.
This product has been referenced in:
- Salazar VS et al. Reactivation of a developmental Bmp2 signaling center is required for therapeutic control of the murine periosteal niche. Elife 8:N/A (2019). Read more (PubMed: 30735122) »
- Coelho JPL et al. A network of chaperones prevents and detects failures in membrane protein lipid bilayer integration. Nat Commun 10:672 (2019). Read more (PubMed: 30737405) »