- Datasheet
- Specific References (54)
- Protocols
Overview
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Product nameAnti-V5 tag antibody [SV5-Pk1]
See all V5 tag primary antibodies -
DescriptionMouse monoclonal [SV5-Pk1] to V5 tag
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Host speciesMouse
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Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Fr, ELISA, IP, WB, RIAmore details
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Immunogen
Mice were infected with the paramyxovirus SV5, Simian-Virus 5.
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Epitopeab27671 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5.
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Positive control
- ICC/IF: CHO cells overexpressing a V5-tagged protein. WB: Mouse cell lysate - whole cell (Cortex cell line).
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General notes
ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityProtein A purified
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Purification notesThis antibody was purified from tissue culture supernatant by affinity chromatography.
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Primary antibody notesAb27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
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ClonalityMonoclonal
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Clone numberSV5-Pk1
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MyelomaSp2/0-Ag14
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IsotypeIgG2a
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
Applications
Our Abpromise guarantee covers the use of ab27671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. PubMed: 21887337 | |
| Flow Cyt | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ELISA | 1/1000 - 1/5000. | |
| IP | Use at an assay dependent concentration. | |
| WB | 1/1000 - 1/5000. | |
| RIA | Use at an assay dependent concentration. |
Target
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RelevanceThe V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
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Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody [SV5-Pk1] (ab27671)](https://www.abcam.com/ps/products/27/ab27671/Images/ab27671-318651-27671444.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody [SV5-Pk1] (ab27671)Benzoubir et al PLoS One. 2015 Jun 25;10(6):e0130559. doi: 10.1371/journal.pone.0130559. eCollection 2015. Fig 1.γSMA is expressed in TGF-β-induced EMT.
HuH7 (Human cell line) cells were transfected with a V5-tagged vector coding for γSMA. Cells were treated with TGF-β for 48h, and the expression of V5, γSMA and αSMA was determined by immunofluorescence analysis. Expression levels of V5 and γSMA or V5 and αSMA were determined by measuring fluorescence intensity on a linear section of the cell.One representative experiment is shown.
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![Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)](https://www.abcam.com/ps/products/27/ab27671/Images/ab27671-318649-27671333.jpg)
Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)Flores et al PLoS One. 2016 Oct 24;11(10):e0165084. doi: 10.1371/journal.pone.0165084. eCollection 2016. Fig S2.Characterization of new C9orf72 dipeptide repeat antibodies.
Rabbit polyclonal antibodies were generated against the sense strand-derived DPRs GA and GR.(A) Western blot of lysates from COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with the indicated vectors. Blots were serially probed with the indicated DPR antibody, then ab27671 and then actin as a loading control.
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![Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody [SV5-Pk1] (ab27671)](https://www.abcam.com/ps/products/27/ab27671/Images/V5-tag-Primary-antibodies-ab27671-6.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-V5 tag antibody [SV5-Pk1] (ab27671)Image courtesy of an anonymous Abreview.ab27671 at a 1/1000 dilution staining V5 tag in CHO (Chinese hamster ovary cell line) cells overexpressing a V5-tagged protein by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton X-100. Samples were blocked with 3% BSA. The secondary used was an Alexa Fluor® 488 conjugated goat anti-mouse IgG (H+L) at a 1/1000 dilution.
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![Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)](https://www.abcam.com/ps/products/27/ab27671/Images/V5-tag-Primary-antibodies-ab27671-5.jpg)
Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)This image is courtesy of an abreview submitted by Melina Reisenberg, CARD, KCL, LondonAnti-V5 tag antibody [SV5-Pk1] (ab27671) at 1/1000 dilution + Mouse cortex cell line lysate at 30 µg
Secondary
HRP-conjugated secondary antibody at 1/3000 dilution
Protocols
References
This product has been referenced in:
- Douse CH et al. Neuropathic MORC2 mutations perturb GHKL ATPase dimerization dynamics and epigenetic silencing by multiple structural mechanisms. Nat Commun 9:651 (2018). Read more (PubMed: 29440755) »
- Helfenberger KE et al. Subcellular distribution of ERK phosphorylation in tyrosine and threonine depends on redox status in murine lung cells. PLoS One 13:e0193022 (2018). Read more (PubMed: 29489891) »
Customer reviews and Q&As
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