For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Mice were infected with the paramyxovirus SV5, Simian-Virus 5.
ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
Our Abpromise guarantee covers the use of ab27671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 21887337|
|Flow Cyt||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ELISA||1/1000 - 1/5000.|
|IP||Use at an assay dependent concentration.|
|WB||1/1000 - 1/5000.|
|RIA||Use at an assay dependent concentration.|
γSMA is expressed in TGF-β-induced EMT.
HuH7 (Human cell line) cells were transfected with a V5-tagged vector coding for γSMA. Cells were treated with TGF-β for 48h, and the expression of V5, γSMA and αSMA was determined by immunofluorescence analysis. Expression levels of V5 and γSMA or V5 and αSMA were determined by measuring fluorescence intensity on a linear section of the cell.
One representative experiment is shown.
Characterization of new C9orf72 dipeptide repeat antibodies.
Rabbit polyclonal antibodies were generated against the sense strand-derived DPRs GA and GR.
(A) Western blot of lysates from COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with the indicated vectors. Blots were serially probed with the indicated DPR antibody, then ab27671 and then actin as a loading control.
ab27671 at a 1/1000 dilution staining V5 tag in CHO (Chinese hamster ovary cell line) cells overexpressing a V5-tagged protein by Immunocytochemistry/ Immunofluorescence.
Cells were fixed in paraformaldehyde and permeabilized using 0.1% Triton X-100. Samples were blocked with 3% BSA. The secondary used was an Alexa Fluor® 488 conjugated goat anti-mouse IgG (H+L) at a 1/1000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"