Anti-V5 tag antibody [SV5-Pk1] (ab27671)
![Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)](https://www.abcam.com/ps/products/27/ab27671/Images/ab27671-380968-anti-v5-tag-antibody-western-blot-nih3t3-cell-lysate.jpg "Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)")
Key features and details
- Mouse monoclonal [SV5-Pk1] to V5 tag
- Suitable for: Flow Cyt, IHC-Fr, ELISA, IP, WB, RIA
- Reacts with: Species independent
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
Anti-V5 tag antibody [SV5-Pk1]
See all V5 tag primary antibodies -
Description
Mouse monoclonal [SV5-Pk1] to V5 tag -
Host species
Mouse -
Tested applications
Suitable for: Flow Cyt, IHC-Fr, ELISA, IP, WB, RIAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Tissue, cells or virus corresponding to V5 tag. Paramyxovirus Simian-Virus 5 (SV5)
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Epitope
ab27671 recognises a small epitope, termed Pk, present on the P/V proteins of the paramyxovirus, SV5. -
Positive control
- WB: Transfected NIH/3T3 and COS-7 cell lysates.
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General notes
ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Purification notes
This antibody was purified from tissue culture supernatant by affinity chromatography. -
Primary antibody notes
Ab27671 has been used to detect recombinant proteins, some of which include transmembrane and secreted proteins, which have been tagged with the V5 epitope. -
Clonality
Monoclonal -
Clone number
SV5-Pk1 -
Myeloma
Sp2/0-Ag14 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use at an assay dependent concentration.
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| IHC-Fr |
Use at an assay dependent concentration.
|
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| ELISA |
1/1000 - 1/5000.
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| IP |
Use at an assay dependent concentration.
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| WB | (8) |
1/1000 - 1/5000.
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| RIA |
Use at an assay dependent concentration.
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| Notes |
|---|
|
Flow Cyt
Use at an assay dependent concentration. |
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IHC-Fr
Use at an assay dependent concentration. |
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ELISA
1/1000 - 1/5000. |
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IP
Use at an assay dependent concentration. |
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WB
1/1000 - 1/5000. |
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RIA
Use at an assay dependent concentration. |
Target
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Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD). -
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Images
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Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)All lanes : Anti-V5 tag antibody [SV5-Pk1] (ab27671) at 1/1000 dilution
Lanes 1-2 : Transfected NIH/3T3 cell lysate
Lane 3 : Not transfected NIH/3T3 cell lysate
Secondary
All lanes : HRP-conjugated rabbit polyclonal Anti-mouse
Developed using the ECL technique.
Exposure time: 1 secondCells were transfected with a V5-tagged HSPB8 (MW:~25kD) plasmid, which expressed a V5-tagged protein with molecular weight of approximately 28kD.
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![Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)](https://www.abcam.com/ps/products/27/ab27671/Images/ab27671-318649-anti-v5-tag-antibody-sv5-pk1-western-blot.jpg)
Western blot - Anti-V5 tag antibody [SV5-Pk1] (ab27671)Flores et al PLoS One. 2016 Oct 24;11(10):e0165084. doi: 10.1371/journal.pone.0165084. eCollection 2016. Fig S2. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/Characterization of new C9orf72 dipeptide repeat antibodies.
Rabbit polyclonal antibodies were generated against the sense strand-derived DPRs GA and GR.(A) Western blot of lysates from COS-7 (African green monkey kidney fibroblast-like cell line) cells transfected with the indicated vectors. Blots were serially probed with the indicated DPR antibody, then ab27671 and then actin as a loading control.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (108)
ab27671 has been referenced in 108 publications.
- Miyoshi T et al. Semi-automated single-molecule microscopy screening of fast-dissociating specific antibodies directly from hybridoma cultures. Cell Rep 34:108708 (2021). PubMed: 33535030
- Rezelj VV et al. Defective viral genomes as therapeutic interfering particles against flavivirus infection in mammalian and mosquito hosts. Nat Commun 12:2290 (2021). PubMed: 33863888
- Xia SL et al. The LRR-TM protein PAN-1 interacts with MYRF to promote its nuclear translocation in synaptic remodeling. Elife 10:N/A (2021). PubMed: 33950834
- Stadler D et al. Interferon-induced degradation of the persistent hepatitis B virus cccDNA form depends on ISG20. EMBO Rep 22:e49568 (2021). PubMed: 33969602
- Gestuveo RJ et al. Analysis of Zika virus capsid-Aedes aegypti mosquito interactome reveals pro-viral host factors critical for establishing infection. Nat Commun 12:2766 (2021). PubMed: 33986255