Product nameAnti-VAC14 antibody - C-terminal
DescriptionRabbit polyclonal to VAC14 - C-terminal
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
- Mouse brain, Rat brain, A431, HeLa, HEK-293T, PC12 and HepG2 cell lysates.
If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 99% PBS
Concentration information loading...
Our Abpromise guarantee covers the use of ab213516 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 88 kDa.|
|ICC/IF||Use a concentration of 8 - 16 µg/ml.|
|IP||Use at an assay dependent concentration.
Use 10-15 μg per reaction.
FunctionThe PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2). Acts as a positive activator of PIKfyve kinase activity. Also required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the VAC14 family.
Contains 6 HEAT repeats.
DomainThe C-terminal domain (residues 523-782) mediates homomeric interactions and is necessary for the formation and maintenance of the PI(3,5)P2 regulatory complex.
Cellular localizationEndosome membrane. Microsome membrane. Mainly associated with membranes of the late endocytic pathway.
- Information by UniProt
- ArPIKfyve antibody
- FLJ10305 antibody
- FLJ36622 antibody
All lanes : Anti-VAC14 antibody - C-terminal (ab213516) at 0.75 µg/ml
Lane 1 : HeLa cell lysate
Lane 2 : HEK-293T cell lysate
Lane 3 : HeLa cell lysate with Human VAC14 immunizing peptide
Lane 4 : HEK-293T cell lysate with Human VAC14 immunizing peptide
Lane 5 : HepG2 cell lysate
Lane 6 : A431 cell lysate
Lane 7 : PC12 cell lysate
Lane 8 : Rat brain (S1 fraction)
Lane 9 : Mouse brain (S1 fraction)
Predicted band size: 88 kDa
VAC14 was immunoprecipitated from A431 cell lysate with ab213516.
Western blot was performed from the immunoprecipitate using ab213516.
Lane 1: IP with ab213516 at 10ug.
Lane 2: IP with ab213516 at 15ug.
Lane 3: Negative control: without ab213516.
Lane 4: Negative control: without cell lysate.
Immunofluorescence analysis of HeLa cells labeling VAC14 with ab213516 at 8µg/ml, followed by Goat Anti-Rabbit IgG, Cy3 conjugate secondary detection (Red). Nuclear staining with DAPI (blue).
ab213516 has not yet been referenced specifically in any publications.