Overview

  • Product name

    Anti-VAC14 antibody - C-terminal
  • Description

    Rabbit polyclonal to VAC14 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide corresponding to Human VAC14 aa 764-782 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    EVRHQRSGRGDHLDRRV VL


    Database link: Q08WQ2

  • Positive control

    • Mouse brain, Rat brain, A431, HeLa, HEK-293T, PC12 and HepG2 cell lysates.
  • General notes

    If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Properties

Applications

Our Abpromise guarantee covers the use of ab213516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 88 kDa.
ICC/IF Use a concentration of 8 - 16 µg/ml.
IP Use at an assay dependent concentration.

Use 10-15 μg per reaction.

Target

  • Function

    The PI(3,5)P2 regulatory complex regulates both the synthesis and turnover of phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2). Acts as a positive activator of PIKfyve kinase activity. Also required to maintain normal levels of phosphatidylinositol 3-phosphate (PtdIns(3)P) and phosphatidylinositol 5-phosphate (PtdIns(5)P). Plays a role in the biogenesis of endosome carrier vesicles (ECV) / multivesicular bodies (MVB) transport intermediates from early endosomes.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the VAC14 family.
    Contains 6 HEAT repeats.
  • Domain

    The C-terminal domain (residues 523-782) mediates homomeric interactions and is necessary for the formation and maintenance of the PI(3,5)P2 regulatory complex.
  • Cellular localization

    Endosome membrane. Microsome membrane. Mainly associated with membranes of the late endocytic pathway.
  • Information by UniProt
  • Database links

  • Alternative names

    • ArPIKfyve antibody
    • FLJ10305 antibody
    • FLJ36622 antibody
    • FLJ46582 antibody
    • MGC149815 antibody
    • MGC149816 antibody
    • OTTHUMP00000174908 antibody
    • Protein VAC14 homolog antibody
    • Tax1 (human T cell leukemia virus type I) binding protein 1 antibody
    • Tax1 (human T cell leukemia virus type I) binding protein 2 antibody
    • Tax1 binding protein 2 antibody
    • Tax1-binding protein 2 antibody
    • TAX1BP2 antibody
    • TRX antibody
    • VAC 14 antibody
    • vac14 antibody
    • Vac14 homolog (S. cerevisiae) antibody
    • Vac14 homolog antibody
    • VAC14_HUMAN antibody
    see all

Images

  • All lanes : Anti-VAC14 antibody - C-terminal (ab213516) at 0.75 µg/ml

    Lane 1 : HeLa cell lysate
    Lane 2 : HEK-293T cell lysate
    Lane 3 : HeLa cell lysate with Human VAC14 immunizing peptide
    Lane 4 : HEK-293T cell lysate with Human VAC14 immunizing peptide
    Lane 5 : HepG2 cell lysate
    Lane 6 : A431 cell lysate
    Lane 7 : PC12 cell lysate
    Lane 8 : Rat brain (S1 fraction)
    Lane 9 : Mouse brain (S1 fraction)

    Predicted band size: 88 kDa

  • VAC14 was immunoprecipitated from A431 cell lysate with ab213516.

    Western blot was performed from the immunoprecipitate using ab213516.

    Lane 1: IP with ab213516 at 10ug.

    Lane 2: IP with ab213516 at 15ug.

    Lane 3: Negative control: without ab213516.

    Lane 4: Negative control: without cell lysate.

  • Immunofluorescence analysis of HeLa cells labeling VAC14 with ab213516 at 8µg/ml, followed by Goat Anti-Rabbit IgG, Cy3 conjugate secondary detection (Red). Nuclear staining with DAPI (blue).

     

     
     
     
     
     

References

ab213516 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab213516.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up