Overview

  • Product name
    Anti-Vaccinia Virus antibody
    See all Vaccinia Virus primary antibodies
  • Description
    Rabbit polyclonal to Vaccinia Virus
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ELISAmore details
  • Species reactivity
    Reacts with vaccinia virus.
  • Immunogen

    Synthetic peptide containing amino acids on the predicted N terminal of fusion protein (A27L) in vaccinia virus.

Properties

Applications

Our Abpromise guarantee covers the use of ab26853 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.

Target

  • Relevance
    Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.
  • Alternative names
    • A27L antibody
    • Orthopoxvirus antibody

Images

  • ELISA method:
    Antigen is coated on EIA strips at 1µg per well.
    Add 200µl blocking buffer then wash wells with PBST.
    Antiserum or peptide specific purified antibody is diluted in series as 102~106 folds.
    Incubate antibody for 1 hour.
    Wash unbound antibodies and add HRP-conjugated anti-rabbit IgG.
    Wash plates and add substrate to develop color for 5 minutes.
    Read absorbance at 650nm. Amount of colour is directly proportional to amount of antibodies.
    Antibody titer is defined as >0.1 of absorbance of antiserum minus pre-immune serum.
    Titer ~ 1/437000.

References

ab26853 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry. I noticed from our records that this had not yet been responded to and I sincerely apologize for the very long delay in this case.

I have been waiting to receive information from our source of this product and I am sorry to confirm that this antibody has not been tested specifically for detection of the D10 protein.

We continually add antibodies and other protein detection products to our catalog but we do not offer custom-made antibodies directly. However, we are happy to be able to offer custom antibody services through Epitomics, an Abcam company which I hope my be helpful on this occasion? For more information about these services visit:

http://www.epitomics.com/services/
email: service@epitomics.com

Alternatively, please contact Li Li at Epitomics:

li.li@epitomics.com

I hope this information is helpful to you. Please do not hesitate to contact us if you have any questions and we will endeavor to get back to you within 24 hours with a response.

Read More

Answer

Thank you for your enquiry and interest in our products.

I can confirm that ab26853 has been tested and characterized only in ELISA, no data available for neutralising assay. Currently, none of our antibodies against Vaccinia Virus have been tested for neutralising assay.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up