Key features and details
- Rabbit polyclonal to Vaccinia Virus
- Suitable for: ELISA
- Reacts with: Vaccinia virus
- Isotype: IgG
Product nameAnti-Vaccinia Virus antibody
See all Vaccinia Virus primary antibodies
DescriptionRabbit polyclonal to Vaccinia Virus
Tested applicationsSuitable for: ELISAmore details
Species reactivityReacts with: Vaccinia virus
Synthetic peptide containing amino acids on the predicted N terminal of fusion protein (A27L) in vaccinia virus.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
Storage bufferConstituent: Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab26853 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceVaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.
- A27L antibody
- Orthopoxvirus antibody
Antigen is coated on EIA strips at 1µg per well.
Add 200µl blocking buffer then wash wells with PBST.
Antiserum or peptide specific purified antibody is diluted in series as 102~106 folds.
Incubate antibody for 1 hour.
Wash unbound antibodies and add HRP-conjugated anti-rabbit IgG.
Wash plates and add substrate to develop color for 5 minutes.
Read absorbance at 650nm. Amount of colour is directly proportional to amount of antibodies.
Antibody titer is defined as >0.1 of absorbance of antiserum minus pre-immune serum.
Titer ~ 1/437000.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab26853 has not yet been referenced specifically in any publications.