Overview

  • Product name

    Anti-Vaccinia Virus antibody
    See all Vaccinia Virus primary antibodies
  • Description

    Rabbit polyclonal to Vaccinia Virus
  • Host species

    Rabbit
  • Specificity

    This antibody reacts with purified Virions. It does not react with uninfected cells.
  • Tested applications

    Suitable for: ELISA, WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Vaccinia virus
  • Immunogen

    Native virus, Lister strain.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • Purification notes

    This antibody is greater than 95% pure.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab35219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/500 - 1/2000.
WB Use at an assay dependent concentration. Predicted molecular weight: 14 kDa. PubMed: 25093734
IHC-P Use at an assay dependent concentration. PubMed: 25093734
IHC-Fr Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 22615950

Target

  • Relevance

    Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.
  • Alternative names

    • A27L antibody
    • Orthopoxvirus antibody

Images

  • All lanes :

    Lane 1 : DT09/06cells infected with GLV-5b451 Vaccina Virus (24 hours post infection)
    Lane 2 : DT09/06cells infected with GLV-5b451 Vaccina Virus (48 hours post infection)
    Lane 3 : DT09/06cells infected with GLV-5b451 Vaccina Virus (72 hours post infection)
    Lane 4 : DT09/06cells infected with GLV-5b451 Vaccina Virus (96 hours post infection)
    Lane 5 : DT09/06 cells infected with LIVP 6.1.1 Vaccina Virus (24 hours post infection)
    Lane 6 : DT09/06 cells infected with LIVP 6.1.1 Vaccina Virus (48 hours post infection)
    Lane 7 : DT09/06 cells infected with LIVP 6.1.1 Vaccina Virus (72 hours post infection)
    Lane 8 : DT09/06 cells infected with LIVP 6.1.1 Vaccina Virus (96 hours post infection)
    Lane 9 : Mock infected DT09/06 cells (24 hours)
    Lane 10 : Mock infected DT09/06 cells (48 hours)
    Lane 11 : Mock infected DT09/06 cells (72 hours)
    Lane 12 : Mock infected DT09/06 cells (96 hours)

    Developed using the ECL technique.

    Predicted band size: 14 kDa



    Data from: PLoS One (2014) 9, e104337.

    Adelfinger, M., Gentschev, I., de Guilbert, J.G.,  Weibel, S., Langnein-Laugwitz, J., Hartl, B., Escobar, H.M., Nolte, I., Chen, N.G., Aguilar, R.J., Yu, Y.A., Zhang, Q., Frentzen, A. and Szalay, A.A.

  • Formalin/PFA-fixed paraffin-embedded mouse cornea stained for Vaccinia Virus with ab35219 at 1/40 dilution in immunohistochemical analysis.

    Heat mediated antigen retrieval was performed in Tris buffer at pH 9.0.

    WR strain of Vaccina Virus.

    See Abreview

References

This product has been referenced in:

  • Warner SG  et al. A Novel Chimeric Poxvirus Encoding hNIS Is Tumor-Tropic, Imageable, and Synergistic with Radioiodine to Sustain Colon Cancer Regression. Mol Ther Oncolytics 13:82-92 (2019). Read more (PubMed: 31061881) »
  • Schuelke MR  et al. Diverse immunotherapies can effectively treat syngeneic brainstem tumors in the absence of overt toxicity. J Immunother Cancer 7:188 (2019). Read more (PubMed: 31315671) »
See all 17 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Cross section of mouse eye, looked at cornea)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS EDTA pH 9
Permeabilization
No
Specification
Cross section of mouse eye, looked at cornea
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
Paraformaldehyde

Ms. Inna Larsen

Verified customer

Submitted Dec 29 2015

Answer


Unfortunately, none of the anti-Vaccinia Virus antibodies we have has been tested for cross-reactivity with Western Reserve Strain.


ab117453 has been tested and cross-reacts with 3 other Vaccinia strains and also with distantly related Monkeypox.

ab28580 does not react with human cellular components.

ab35219 is known to be reactive to the Lister and MVA strains of Vaccinia. It only binds to the virus components and does not react to uninfected cells.

ab156854 has only been tested in the Lister and NYCBH strains. This antibody does not react with human cellular components.

Read More

Answer

Thank you for contacting us.

We only have two monoclonal antibodies against the vaccinia virus in our catalogue.

I would recommend using ab21098 - Mouse monoclonal [TV46] to Vaccinia Virus (https://www.abcam.com/ab21098) to recognise A27L.

I have not found an antibody against H3L and D8L in our catalogue. I would like to recommend checking the Biocompare website which has an excellent antibody search facility that includes many suppliers. The links are:
www.biocompare.com
http://www.biocompare.com/ProductCategories/2045/Antibodies.html

As for the antibody that will neutralize the purified forms of the vaccinia virus we do not have any antibodies tested as neutralising antibodies. However, if you would like to buy and test either one of our mouse monoclonal antibodies (ab48569 or ab21098) as a neutralising antibody, we can offer you a discount off your next order.

Simply follow these easy steps below to apply for our AbTrial Program:

1. Reply to this email, letting us know you are interested in testing this product.

2. Our scientists will email you an inactive personal discount code for the value of the product.

3. Purchase and test the product at the regular price.

4. Submit your results, including your discount code in the additional notes section of your Abreview.

5. Once the Abreview is submitted, the discount code will become active.

6. Apply your discount code on your next order to receive that value off.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/abtrial.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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