Overview

  • Product name

    Anti-Vaccinia Virus antibody (Biotin)
    See all Vaccinia Virus primary antibodies
  • Description

    Rabbit polyclonal to Vaccinia Virus (Biotin)
  • Host species

    Rabbit
  • Conjugation

    Biotin
  • Tested applications

    Suitable for: ELISAmore details
  • Species reactivity

    Reacts with: Vaccinia virus
  • Immunogen

    Lister Strain (mixture of virions and infected cell polypeptides).

  • General notes

    The antibody is covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab21039 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Target

  • Relevance

    Vaccinia virus is an Orthopoxvirus, containing double stranded DNA. Fusion protein plays an important role in the entry of enveloped virus into cells. As vaccinia virus has a wide host range, it is conceivable that certain cellular components that are ubiquitously expressed on the cell mediate virus infection. The study of the entry process, attachment, fusion and the proteins and receptors involved is complex. During vaccinia virus infection, the fusion process is attributed to the action of the 14KDa protein (A27L). The N terminus of this protein recognises heparan sulfate on the cell surface. It interacts with the negative charges of sulfates of glycosaminoglycans (GAGs). Therefore, antibodies that recognize this 14KDa protein are able to neutralize vaccinia virus infection and enable identification other viral and cellular proteins which participate in the vaccinia virus entry process.
  • Alternative names

    • A27L antibody
    • Orthopoxvirus antibody

References

This product has been referenced in:

  • Ylösmäki E  et al. Personalized Cancer Vaccine Platform for Clinically Relevant Oncolytic Enveloped Viruses. Mol Ther 26:2315-2325 (2018). Read more (PubMed: 30005865) »
See 1 Publication for this product

Customer reviews and Q&As

Answer

Thank you for your enquiry. Ab21098 - Mouse monoclonal [TV46] to Vaccinia Virus - reacts with a 27 kDa protein and is weakly reactive with 57 kDa and 6 1kDa proteins in Western blot. The identity of the proteins have not been determined. Ab21039 - Rabbit polyclonal to Vaccinia Virus (Biotin) - is a polyclonal antibody and will detect multiple epitopes. The individual proteins have not been identified. This antibody reacts to Lister, Wyeth, New York City and MVA strains of Vaccinia virus. Ab20041 is no longer available as part of our catalog. Please contact us again if you have any additional questions.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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