Overview

  • Product name

  • Description

    Rabbit polyclonal to VAChT
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • ICC/IF: Post-natal rat pup (PND1) heterogeneous brain cells. IHC-P: Rat spinal cord tissue. WB: Post-natal rat pup whole brain lysate (PND2-6)
  • General notes

     This product was previously labelled as Vesicular Acetylcholine Transporter

     

Properties

Applications

Our Abpromise guarantee covers the use of ab235201 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 56 kDa.
ICC/IF Use a concentration of 5 - 15 µg/ml.
IHC-P 1/100.

Antigen retrieval: HIER using citrate buffer for 20 minutes.

Target

Images

  • 4% PFA-fixed, 0.3%Triton X-100 permeabilized Post-natal rat pup (PND1) heterogeneous brain cells labeling VAChT
    (green) using ab235201 at 15 μg/mL, O/N at 4⁰C. Donkey Anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5μg/ml for 1 h at RT. The nuclear counterstain was DAPI (blue).

    Expected localization: predicted membrane, golgi apparatus

    Antibody detecting dendrites and axons and specific organelles in the nucleus (nucleolus). Staining correspondent to confirmed VAChT (Slc18a3) staining in mouse brain P7 Gensat project:

    http://www.gensat.org/imagenavigator.jsp?imageID=66611

    (A) DAPI

    (B) ab235201 + secondary antibody

    (C) Merge images A+B.

    (D) Secondary antibody only

  • Anti-VAChT antibody (ab235201) at 1/1000 dilution + Post-natal rat pup whole brain lysate (PND2-6)

    Predicted band size: 56 kDa

  • Paraffin-embedded rat spinal cord tissue stained for VAChT using ab235201 at 1/100 dilution for 30 minutes at room temperature. Secondary antibody was an anti-Rabbit Poly-HRP-IgG, ready to use for 8 minutes at room temperature. Counterstained with hematoxylin.

    A: Rat spinal cord H&E.

    B: ab235201 20X magnification.

    C: ab235201 40X magnification.

References

ab235201 has not yet been referenced specifically in any publications.

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