Product nameAnti-VAChT antibody
See all VAChT primary antibodies
DescriptionRabbit polyclonal to VAChT
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Rat
Predicted to work with: Mouse, Human
Synthetic peptide. This information is considered to be commercially sensitive.
- ICC/IF: Post-natal rat pup (PND1) heterogeneous brain cells. IHC-P: Rat spinal cord tissue. WB: Post-natal rat pup whole brain lysate (PND2-6)
This product was previously labelled as Vesicular Acetylcholine Transporter
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235201 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 56 kDa.|
|ICC/IF||Use a concentration of 5 - 15 µg/ml.|
Antigen retrieval: HIER using citrate buffer for 20 minutes.
FunctionInvolved in acetylcholine transport into synaptic vesicles.
Tissue specificityPeripheral and central cholinergic nervous systems.
Sequence similaritiesBelongs to the major facilitator superfamily. Vesicular transporter family.
- Information by UniProt
- AChR antibody
- MGC12716 antibody
- rVAT antibody
4% PFA-fixed, 0.3%Triton X-100 permeabilized Post-natal rat pup (PND1) heterogeneous brain cells labeling VAChT
(green) using ab235201 at 15 μg/mL, O/N at 4⁰C. Donkey Anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed was used as the secondary antibody at 5μg/ml for 1 h at RT. The nuclear counterstain was DAPI (blue).
Expected localization: predicted membrane, golgi apparatus
Antibody detecting dendrites and axons and specific organelles in the nucleus (nucleolus). Staining correspondent to confirmed VAChT (Slc18a3) staining in mouse brain P7 Gensat project:
(B) ab235201 + secondary antibody
(C) Merge images A+B.
(D) Secondary antibody only
Anti-VAChT antibody (ab235201) at 1/1000 dilution + Post-natal rat pup whole brain lysate (PND2-6)
Predicted band size: 56 kDa
Paraffin-embedded rat spinal cord tissue stained for VAChT using ab235201 at 1/100 dilution for 30 minutes at room temperature. Secondary antibody was an anti-Rabbit Poly-HRP-IgG, ready to use for 8 minutes at room temperature. Counterstained with hematoxylin.
A: Rat spinal cord H&E.
B: ab235201 20X magnification.
C: ab235201 40X magnification.
ab235201 has not yet been referenced specifically in any publications.