Overview

  • Product name
  • Description
    Sheep polyclonal to VAChT
  • Host species
    Sheep
  • Specificity

    Ab31544 is specific to VAChT.

  • Tested applications
    Suitable for: IHC - Wholemount, WBmore details
  • Species reactivity
    Reacts with: Rat, Guinea pig
    Predicted to work with: Mouse, Dog, Human, Chimpanzee, Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Rat VAChT aa 475-500.
    Sequence:

    C-TRSRSERDVLLDEPPQGLYDAVRLRE

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab31544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC - Wholemount Use at an assay dependent concentration. Fix Tissue overnight in Zamboni fixative, then carry out 3 x 10min washes in DMSO and 3 x 10min washes in PBS. Tissue was then stored in PBS and Azide until being dissected and stained.
WB Use at an assay dependent dilution. Predicted molecular weight: 59 kDa.

Target

References

ab31544 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Tissue lysate - whole (Lung)
Gel Running Conditions
Reduced Denaturing
Loading amount
30 µg
Specification
Lung
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Dr. Safa Azar

Verified customer

Submitted Sep 13 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (parasympathetic nerve fibres)
Specification
parasympathetic nerve fibres
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Aug 28 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - 0.1% Tween 20
Specification
Spinal cord
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Mrs. Teresa Garrett

Verified customer

Submitted Oct 14 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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