Overview

  • Product name

    Valproic acid, sodium salt, Histone deacetylase inhibitor
  • Description

    Histone deacetylase inhibitor
  • Alternative names

    • 2-propylpentanoic acid sodium
    • Sodium 2-propylpentanoate
    • Sodium valproate
    • Valproate sodium
  • Biological description

    Histone deacetylase inhibitor (IC50 = 0.4 μM for HDAC1). Increases GABA expression and activates Wnt dependent gene expression. Centrally active upon oral and intravenous administration.
  • CAS Number

    1069-66-5
  • Chemical structure

    Chemical Structure

Properties

Applications

Our Abpromise guarantee covers the use of ab120745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab70362 staining adiponectin receptor 1 in HepG2 cells treated with valproic acid, sodium salt (ab120745), by ICC/IF. Increased cytoplasmatic staining of adiponectin receptor 1 correlates with increased concentration of valproic acid, as described in literature. The cells were incubated at 37°C for 24h in media containing different concentrations of ab120745 (valproic acid) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab70362 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

References

This product has been referenced in:

  • De Sarno P  et al. Regulation of Akt and glycogen synthase kinase-3 beta phosphorylation by sodium valproate and lithium. Neuropharmacology 43:1158-64 (2002). Read more (PubMed: 12504922) »
  • Phiel CJ  et al. Histone deacetylase is a direct target of valproic acid, a potent anticonvulsant, mood stabilizer, and teratogen. J Biol Chem 276:36734-41 (2001). Read more (PubMed: 11473107) »
  • Keane PE  et al. The effects of analogues of valproic acid on seizures induced by pentylenetetrazol and GABA content in brain of mice. Neuropharmacology 22:875-9 (1983). Read more (PubMed: 6413882) »

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Unfortunately, we do not conduct scientific research within our labs and so we do not test the specific actions of our inhibitors. We simply produce this biochemical to a high quality.

I would suggest that your customer looks at the available literature for Valproic acid to see what other researchers have found through their research.

From my brief literature research, I have found that valproic acid inhibits both class I and II HDACs, with a high potency for class I HDACs.

Please see the following publication which may also be of interest to your customer:

Valproic acid defines a novel class of HDAC inhibitors inducing differentiation of transformed cells
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC125788/

Read More

Answer

Thank you for contacting us.

Yes, I can confirm that the ab40988 is able to recognize free valproic acid, therefore the ab120745. The laboratory confirmed indeed that this antibody has been used in a competitive immunoassay in which antibody binding to fluorogenic valproic acid is inhibited by the presence of free valproic acid.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
For licensing inquiries, please contact partnerships@abcam.com

Sign up