Product nameAnti-valyl tRNA synthetase antibody [EPR11300] - C-terminal
See all valyl tRNA synthetase primary antibodies
DescriptionRabbit monoclonal [EPR11300] to valyl tRNA synthetase - C-terminal
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human valyl tRNA synthetase aa 1250 to the C-terminus. The exact sequence is proprietary.
Database link: P26640
- HepG2, HeLa, Ramos and K562 cell lysates; Human kidney tissue; HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/100.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/1000 - 1/10000. Detects a band of approximately 140 kDa (predicted molecular weight: 140 kDa).|
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 GST C-terminal domain.
- Information by UniProt
- Bat 6 antibody
- Bat6 antibody
- D17H6S56E antibody
All lanes : Anti-valyl tRNA synthetase antibody [EPR11300] - C-terminal (ab181175) at 1/5000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Ramos cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution
Developed using the ECL technique.
Predicted band size: 140 kDa
Observed band size: 140 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling valyl tRNA synthetase with ab181175 at 1/100 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescent analysis of HepG2 cells (paraformaldehyde-fixed, 4%) labeling valyl tRNA synthetase with ab181175 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary at 1/200 dilution and counter-stained with DAPI (blue).
ab181175 has not yet been referenced specifically in any publications.