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RecombinantRabMAb

Recombinant Anti-VAM1 antibody [EPR14157(B)] (ab180508)

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  • SDS
  • Certificate of Compliance
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Immunoprecipitation - Anti-VAM1 antibody [EPR14157(B)] (ab180508)
  • Western blot - Anti-VAM1 antibody [EPR14157(B)] (ab180508)
  • Anti-VAM1 antibody [EPR14157(B)] (ab180508)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14157(B)] to VAM1
  • Suitable for: WB, IP
  • Reacts with: Human

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-VAM1 antibody [EPR14157(B)]
    See all VAM1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14157(B)] to VAM1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293, HepG2, NTERA2/D1 and Human fetal brain lysates.
  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR14157(B)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Other

Associated products

  • Alternative Versions

    • Anti-VAM1 antibody [EPR14157(B)] - BSA and Azide free (ab250210)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 whole cell lysate (ab166833)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IP
1/60.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IP
1/60.

Target

  • Tissue specificity

    Abundant in testis, brain, and kidney with lower levels detectable in other tissues.
  • Sequence similarities

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 2 L27 domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SH3 domain.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q9NZW5 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 51678 Human
    • Entrez Gene: 56524 Mouse
    • Entrez Gene: 362359 Rat
    • Omim: 606959 Human
    • SwissProt: Q9NZW5 Human
    • SwissProt: Q9JLB0 Mouse
    • Unigene: 533355 Human
    • Unigene: 248361 Mouse
    • Unigene: 41288 Mouse
    • Unigene: 463873 Mouse
    see all
  • Alternative names

    • MAGUK p55 subfamily member 6 antibody
    • MAGUK p55 subfamily member 6 VELI associated MAGUK 1 antibody
    • MAGUK protein p55T antibody
    • Membrane protein palmitoylated 6 antibody
    • Mpp6 antibody
    • MPP6_HUMAN antibody
    • p55T antibody
    • Palmitoylated 6 MAGUK p55 subfamily member 6 antibody
    • PALS2 antibody
    • Protein associated with Lin7 2 antibody
    • VAM-1 antibody
    • VELI associated MAGUK 1 antibody
    • Veli-associated MAGUK 1 antibody
    see all

Images

  • Immunoprecipitation - Anti-VAM1 antibody [EPR14157(B)] (ab180508)
    Immunoprecipitation - Anti-VAM1 antibody [EPR14157(B)] (ab180508)

    Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab180508 at 1/60 dilution.
    Secondary: Anti-Rabbit IgG HRP at 1/1500 dilution.

  • Western blot - Anti-VAM1 antibody [EPR14157(B)] (ab180508)
    Western blot - Anti-VAM1 antibody [EPR14157(B)] (ab180508)
    All lanes : Anti-VAM1 antibody [EPR14157(B)] (ab180508) at 1/5000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : NTERA2/D1 cell lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 61 kDa



    Blocking buffer: 5% NFDM/TBST

  • Anti-VAM1 antibody [EPR14157(B)] (ab180508)
    Anti-VAM1 antibody [EPR14157(B)] (ab180508)

Protocols

  • Western blot protocols
  • Immunoprecipitation protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (0)

Publishing research using ab180508? Please let us know so that we can cite the reference in this datasheet.

ab180508 has not yet been referenced specifically in any publications.

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