RecombinantRabMAb

Recombinant Anti-VAM1 antibody [EPR14157(B)] (ab180508)

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Immunoprecipitation - Anti-VAM1 antibody [EPR14157(B)] (ab180508)
  • Western blot - Anti-VAM1 antibody [EPR14157(B)] (ab180508)
  • Anti-VAM1 antibody [EPR14157(B)] (ab180508)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14157(B)] to VAM1
  • Suitable for: WB, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-VAM1 antibody [EPR14157(B)]
    See all VAM1 primary antibodies

  • Description

    Rabbit monoclonal [EPR14157(B)] to VAM1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293, HepG2, NTERA2/D1 and Human fetal brain lysates.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab180508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IP
1/60.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IP
1/60.

Target

  • Tissue specificity

    Abundant in testis, brain, and kidney with lower levels detectable in other tissues.

  • Sequence similarities

    Belongs to the MAGUK family.
    Contains 1 guanylate kinase-like domain.
    Contains 2 L27 domains.
    Contains 1 PDZ (DHR) domain.
    Contains 1 SH3 domain.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • MAGUK p55 subfamily member 6 antibody
    • MAGUK p55 subfamily member 6 VELI associated MAGUK 1 antibody
    • MAGUK protein p55T antibody
    • Membrane protein palmitoylated 6 antibody
    • Mpp6 antibody
    • MPP6_HUMAN antibody
    • p55T antibody
    • Palmitoylated 6 MAGUK p55 subfamily member 6 antibody
    • PALS2 antibody
    • Protein associated with Lin7 2 antibody
    • VAM-1 antibody
    • VELI associated MAGUK 1 antibody
    • Veli-associated MAGUK 1 antibody
    see all

Images

  • Immunoprecipitation - Anti-VAM1 antibody [EPR14157(B)] (ab180508)
    Immunoprecipitation - Anti-VAM1 antibody [EPR14157(B)] (ab180508)

    Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab180508 at 1/60 dilution.
    Secondary: Anti-Rabbit IgG HRP at 1/1500 dilution.

  • Western blot - Anti-VAM1 antibody [EPR14157(B)] (ab180508)
    Western blot - Anti-VAM1 antibody [EPR14157(B)] (ab180508)
    All lanes : Anti-VAM1 antibody [EPR14157(B)] (ab180508) at 1/5000 dilution

    Lane 1 : 293 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : NTERA2/D1 cell lysate
    Lane 4 : Human fetal brain lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 61 kDa



    Blocking buffer: 5% NFDM/TBST

  • Anti-VAM1 antibody [EPR14157(B)] (ab180508)
    Anti-VAM1 antibody [EPR14157(B)] (ab180508)

References (0)

Publishing research using ab180508? Please let us know so that we can cite the reference in this datasheet.

ab180508 has not yet been referenced specifically in any publications.

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