Recombinant Anti-VAM1 antibody [EPR14157(B)] (ab180508)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-VAM1 antibody [EPR14157(B)]
See all VAM1 primary antibodies -
Description
Rabbit monoclonal [EPR14157(B)] to VAM1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human VAM1 aa 1-100. The exact sequence is proprietary.
Database link: Q9NZW5 -
Positive control
- 293, HepG2, NTERA2/D1 and Human fetal brain lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR14157(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab180508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). | |
| IP | 1/60. |
Target
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Tissue specificity
Abundant in testis, brain, and kidney with lower levels detectable in other tissues. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 2 L27 domains.
Contains 1 PDZ (DHR) domain.
Contains 1 SH3 domain. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 51678 Human
- Entrez Gene: 56524 Mouse
- Entrez Gene: 362359 Rat
- Omim: 606959 Human
- SwissProt: Q9NZW5 Human
- SwissProt: Q9JLB0 Mouse
- Unigene: 533355 Human
- Unigene: 248361 Mouse
see all -
Alternative names
- MAGUK p55 subfamily member 6 antibody
- MAGUK p55 subfamily member 6 VELI associated MAGUK 1 antibody
- MAGUK protein p55T antibody
see all
Images
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![Immunoprecipitation - Anti-VAM1 antibody [EPR14157(B)] (ab180508)](https://www.abcam.com/ps/products/180/ab180508/Images/ab180508-215619-anti-vam1-antibody-epr14157b-immunoprecipitation.jpg)
Immunoprecipitation - Anti-VAM1 antibody [EPR14157(B)] (ab180508)Western blot analysis of immunoprecipitation pellet from 293 cell lysate immunoprecipitated using ab180508 at 1/60 dilution.
Secondary: Anti-Rabbit IgG HRP at 1/1500 dilution. -
![Western blot - Anti-VAM1 antibody [EPR14157(B)] (ab180508)](https://www.abcam.com/ps/products/180/ab180508/Images/ab180508-215617-anti-vam1-antibody-epr14157b-western-blot.jpg)
Western blot - Anti-VAM1 antibody [EPR14157(B)] (ab180508)All lanes : Anti-VAM1 antibody [EPR14157(B)] (ab180508) at 1/5000 dilution
Lane 1 : 293 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : NTERA2/D1 cell lysate
Lane 4 : Human fetal brain lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 61 kDaBlocking buffer: 5% NFDM/TBST
Datasheets and documents
Certificate of Compliance
References
ab180508 has not yet been referenced specifically in any publications.
