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Rabbit polyclonal to VAMP1
Predicted to work with:
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat VAMP1.
(Peptide available as
WB: This antibody gave a positive signal in the following tissue lysates:
Mouse Brain, Mouse Spinal Cord, Rat Brain and Rat Spinal Cord.
IHC: rat brain
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Immunizing Peptide (Blocking)
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 1 mg/ml. Detects a band of approximately 17 kDa (predicted molecular weight: 13 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Involved in the targeting and/or fusion of transport vesicles to their target membrane.
Nervous system, skeletal muscle and adipose tissue.
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Mitochondrion outer membrane; Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome and Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome.
Information by UniProt
SYB 1 antibody
Western blot - Anti-VAMP1 antibody (ab41324)
All lanes :
Anti-VAMP1 antibody (ab41324) at 1 µg/ml
Lane 1 :
Brain (Mouse) Tissue Lysate
Lane 2 :
Brain (Rat) Tissue Lysate - normal tissue
Lane 3 :
Spinal Cord (Mouse) Tissue Lysate
Lane 4 :
Spinal Cord (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size:
Observed band size:
17 kDa (
why is the actual band size different from the predicted?
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-VAMP1 antibody (ab41324) This image is courtesy of an abreview submitted by Sophie Pezet, CNRS, Paris, France
Immunohistochemistical detection of VAMP1 with ab41324 on rat brain PFA perfusion-fixed frozen sections. Primary antibody ab41324 diluted at 1/3000 and incubated for 18 hours at 20°C in PBS + 0.3 % Triton X100. Secondary antibody: goat anti-rabbit Alexa Fluor 488 (1/1000)Immunostaining obtained in the rat hippocampus using this antibody diluted 1:1000. Sections come from a perfused-fixed animal. The immunostaining was performed using the `free floating` technique, using direct fluorescence. The antibody stains synapses in many brain areas. The picture shows the staining taken at low power magnification, showing the staining in the hippocampus (top) and medium thalamus (bottom of the picture).
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"