Overview

  • Product name
    Anti-VAMP1 antibody [EPR7325(2)]
    See all VAMP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7325(2)] to VAMP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide, corresponding to residues in Human VAMP1 (UniProt: P23763).

  • Positive control
    • Human cerebellum lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab151712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Involved in the targeting and/or fusion of transport vesicles to their target membrane.
    • Tissue specificity
      Nervous system, skeletal muscle and adipose tissue.
    • Sequence similarities
      Belongs to the synaptobrevin family.
      Contains 1 v-SNARE coiled-coil homology domain.
    • Cellular localization
      Mitochondrion outer membrane; Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome and Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome.
    • Information by UniProt
    • Database links
    • Alternative names
      • DKFZp686H12131 antibody
      • SYB 1 antibody
      • SYB1 antibody
      • Synaptobrevin 1 antibody
      • Synaptobrevin-1 antibody
      • Synaptobrevin1 antibody
      • VAMP 1 antibody
      • VAMP-1 antibody
      • Vamp1 antibody
      • VAMP1_HUMAN antibody
      • Vesicle associated membrane protein 1 antibody
      • Vesicle associated membrane protein 1 synaptobrevin 1 antibody
      • Vesicle-associated membrane protein 1 antibody
      see all

    Images

    • Anti-VAMP1 antibody [EPR7325(2)] (ab151712) at 1/1000 dilution + Human cerebellum lysate at 10 µg

      Predicted band size: 13 kDa

    • Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab151712 showing +ve staining.
    • Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab151712 showing -ve staining.
    • Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab151712 showing -ve staining.
    • Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab151712 showing -ve staining.
    • Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151712 showing -ve staining.

    References

    This product has been referenced in:
    • Her LS  et al. miR-196a Enhances Neuronal Morphology through Suppressing RANBP10 to Provide Neuroprotection in Huntington's Disease. Theranostics 7:2452-2462 (2017). WB ; Mouse . Read more (PubMed: 28744327) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (primary culture hippocampus cell)
    Gel Running Conditions
    Non-reduced Denaturing (15% SDS-PAGE)
    Loading amount
    15 µg
    Treatment
    RIPA & 1%Protease inhibitor for 15min on ice
    Specification
    primary culture hippocampus cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

    Abcam user community

    Verified customer

    Submitted Oct 10 2018

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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