Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
RecombinantRabMAb

Recombinant Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

Reviews (1) Submit a question References (1)
Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
  • Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7325(2)] to VAMP1
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

Overview

  • Product name

    Anti-VAMP1 antibody [EPR7325(2)]
    See all VAMP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR7325(2)] to VAMP1

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse brain, Rat brain, Human cerebellum lysate. IHC-P: Human brain

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab151712 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 13 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Application notes
Is unsuitable for ICC/IF or IP.

Target

  • Function

    Involved in the targeting and/or fusion of transport vesicles to their target membrane.

  • Tissue specificity

    Nervous system, skeletal muscle and adipose tissue.

  • Sequence similarities

    Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.

  • Cellular localization

    Mitochondrion outer membrane; Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome and Cytoplasmic vesicle membrane. Cell junction > synapse > synaptosome.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • DKFZp686H12131 antibody
    • SYB 1 antibody
    • SYB1 antibody
    • Synaptobrevin 1 antibody
    • Synaptobrevin-1 antibody
    • Synaptobrevin1 antibody
    • VAMP 1 antibody
    • VAMP-1 antibody
    • Vamp1 antibody
    • VAMP1_HUMAN antibody
    • Vesicle associated membrane protein 1 antibody
    • Vesicle associated membrane protein 1 synaptobrevin 1 antibody
    • Vesicle-associated membrane protein 1 antibody
    see all

Images

  • Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    All lanes : Anti-VAMP1 antibody [EPR7325(2)] (ab151712) at 1/1000 dilution

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Rat brain tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 13 kDa
    Observed band size: 16 kDa why is the actual band size different from the predicted?



    Blocking and diluting buffer and concentration: 5% NFDM/TBST

    Exposure time: 10 seconds

  • Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Western blot - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Anti-VAMP1 antibody [EPR7325(2)] (ab151712) at 1/1000 dilution + Human cerebellum lysate at 10 µg

    Predicted band size: 13 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded normal Human brain tissue using ab151712 showing +ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab151712 showing -ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab151712 showing -ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded Human Skeletal muscle tissue using ab151712 showing -ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

    Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab151712 showing -ve staining.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Anti-VAMP1 antibody [EPR7325(2)] (ab151712)
    Anti-VAMP1 antibody [EPR7325(2)] (ab151712)

References (1)

Publishing research using ab151712? Please let us know so that we can cite the reference in this datasheet.

ab151712 has been referenced in 1 publication.

  • Her LS  et al. miR-196a Enhances Neuronal Morphology through Suppressing RANBP10 to Provide Neuroprotection in Huntington's Disease. Theranostics 7:2452-2462 (2017). WB ; Mouse . PubMed: 28744327

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review

Filter by Application

Filter by Species

Filter by Ratings

Western blot abreview for Anti-VAMP1 antibody [EPR7325(2)]

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (primary culture hippocampus cell)
Gel Running Conditions
Non-reduced Denaturing (15% SDS-PAGE)
Loading amount
15 µg
Treatment
RIPA & 1%Protease inhibitor for 15min on ice
Specification
primary culture hippocampus cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Oct 10 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com