Product nameAnti-VAMP2 antibody
See all VAMP2 primary antibodies
DescriptionRabbit polyclonal to VAMP2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Cow, Human, Xenopus laevis, Zebrafish, Rhesus monkey
Recombinant full length protein within Human VAMP2. The exact sequence is proprietary.
Database link: P63027
- WB: Mouse brain, rat brain tissue extracts. ICC/IF: DIV9 rat E18 primary cortical neurons.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 13 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in the targeting and/or fusion of transport vesicles to their target membrane.
Tissue specificityNervous system and skeletal muscle.
Sequence similaritiesBelongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
- Information by UniProt
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
All lanes : Anti-VAMP2 antibody (ab228996) at 1/1000 dilution
Lane 1 : Mouse brain tissue extract.
Lane 2 : Rat brain tissue extract.
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 13 kDa
15% SDS-PAGE gel.
Paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for VAMP2 protein (green) using ab28996 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Fluoroshield with Dapi (blue).
Beta Tubulin 3/ Tuj1 (red).
ab228996 has not yet been referenced specifically in any publications.