Key features and details
- Rabbit polyclonal to VAMP2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Product nameAnti-VAMP2 antibody
See all VAMP2 primary antibodies
DescriptionRabbit polyclonal to VAMP2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat
Predicted to work with: Cow, Human, Xenopus laevis, Zebrafish, Rhesus monkey
Recombinant full length protein within Human VAMP2. The exact sequence is proprietary.
Database link: P63027
- WB: Mouse brain, rat brain tissue extracts. ICC/IF: DIV9 rat E18 primary cortical neurons.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228996 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 13 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in the targeting and/or fusion of transport vesicles to their target membrane.
Tissue specificityNervous system and skeletal muscle.
Sequence similaritiesBelongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
- Information by UniProt
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
All lanes : Anti-VAMP2 antibody (ab228996) at 1/1000 dilution
Lane 1 : Mouse brain tissue extract.
Lane 2 : Rat brain tissue extract.
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 13 kDa
15% SDS-PAGE gel.
Paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons stained for VAMP2 protein (green) using ab28996 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Fluoroshield with Dapi (blue).
Beta Tubulin 3/ Tuj1 (red).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228996 has not yet been referenced specifically in any publications.