Anti-VAMP2 antibody (ab3347)
Key features and details
- Rabbit polyclonal to VAMP2
- Suitable for: ICC/IF, IHC-FoFr, IHC-Fr, WB, IP
- Reacts with: Mouse, Rat, Cow, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-VAMP2 antibody
See all VAMP2 primary antibodies -
Description
Rabbit polyclonal to VAMP2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-FoFr, IHC-Fr, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Cow, Human
Predicted to work with: Rhesus monkey
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Immunogen
Synthetic peptide corresponding to Rat VAMP2 aa 1-18.
Sequence:MSATAATVPPAAPAGEGG
(Peptide available asab4958) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (1) |
Use at an assay dependent concentration.
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| IHC-FoFr |
Use at an assay dependent concentration. PubMed: 23056393
|
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| IHC-Fr | (1) |
Use at an assay dependent concentration.
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| WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
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| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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IHC-FoFr
Use at an assay dependent concentration. PubMed: 23056393 |
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IHC-Fr
Use at an assay dependent concentration. |
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WB
Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa). |
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IP
Use at an assay dependent concentration. |
Target
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Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane. -
Tissue specificity
Nervous system and skeletal muscle. -
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles. - Information by UniProt
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Database links
- Entrez Gene: 282116 Cow
- Entrez Gene: 6844 Human
- Entrez Gene: 22318 Mouse
- Entrez Gene: 24803 Rat
- Omim: 185881 Human
- SwissProt: P63027 Human
- SwissProt: P63044 Mouse
- SwissProt: P63045 Rat
see all -
Alternative names
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
see all
Images
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Western blot - Anti-VAMP2 antibody (ab3347)All lanes : Anti-VAMP2 antibody (ab3347) at 1 µg/ml
Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Mouse lung lysate
Lane 4 : Rat lung lysate
Lane 5 : Rat liver lysate
Lane 6 : Rat kidney lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 1/4000 dilution
Predicted band size: 19 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (43)
ab3347 has been referenced in 43 publications.
- Wilhelmi I et al. The ARFRP1-dependent Golgi scaffolding protein GOPC is required for insulin secretion from pancreatic ß-cells. Mol Metab 45:101151 (2021). PubMed: 33359402
- Ivanova D et al. Control of synaptic vesicle release probability via VAMP4 targeting to endolysosomes. Sci Adv 7:N/A (2021). PubMed: 33931449
- Rong X et al. Enalapril attenuates endoplasmic reticulum stress and mitochondrial injury induced by myocardial infarction via activation of the TAK1/NFAT pathway in mice. Exp Ther Med 19:972-980 (2020). PubMed: 32010259
- Tanaka M et al. Enhanced phosphorylation of PERK in primary cultured neurons as an autonomous neuronal response to prion infection. PLoS One 15:e0234147 (2020). PubMed: 32479530
- Ke YD et al. CNS cell type-specific gene profiling of P301S tau transgenic mice identifies genes dysregulated by progressive tau accumulation. J Biol Chem 294:14149-14162 (2019). PubMed: 31366728