Product nameAnti-VAMP2 antibody
See all VAMP2 primary antibodies
DescriptionRabbit polyclonal to VAMP2
Tested applicationsSuitable for: WB, IP, IHC-Fr, ICC/IF, IHC-FoFrmore details
Species reactivityReacts with: Mouse, Rat, Cow, Human
Predicted to work with: Rhesus monkey
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab3347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).Can be blocked with VAMP2 peptide (ab4958).|
|IP||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC-FoFr||Use at an assay dependent concentration. PubMed: 23056393|
FunctionInvolved in the targeting and/or fusion of transport vesicles to their target membrane.
Tissue specificityNervous system and skeletal muscle.
Sequence similaritiesBelongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
- Information by UniProt
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
Western blot detection of VAMP2 on rat brain extract using ab3347.
VAMP2 antibody (ab3347) used in Immunohistochemistry (rat frozen sections). Sections were fixed with paraformaldehyde. Primary Antibody ab3347 used at 1/1000 incubated for 18 hours at 20°C in PBS-T 0.3%. Secondary Antibody used anti-IgG Rabbit Conjugation Alexa Fluor® 488 Dilution 1/1000.
Immunohistochemical analysis of rat spinal cord dorsal horn tissue, staining VAMP2 with ab3347 at 1/200 dilution. Fluorescent-conjugated anti-rabbit IgG (1/200) was used as the secondary antibody.
This product has been referenced in:
- Alexander NJ et al. SNARE Complex-Associated Proteins in the Lateral Amygdala of Macaca mulatta Following Long-Term Ethanol Drinking. Alcohol Clin Exp Res 42:1661-1673 (2018). Read more (PubMed: 29944190) »
- Kim D et al. Graphene quantum dots prevent a-synucleinopathy in Parkinson's disease. Nat Nanotechnol N/A:N/A (2018). Read more (PubMed: 29988049) »