- Datasheet
- Specific References (22)
- Protocols
Overview
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Product nameAnti-VAMP2 antibody
See all VAMP2 primary antibodies -
DescriptionRabbit polyclonal to VAMP2
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-Fr, ICC/IF, IHC-FoFrmore details
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Species reactivityReacts with: Mouse, Rat, Cow, Human
Predicted to work with: Rhesus monkey
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Immunogen
Synthetic peptide corresponding to Rat VAMP2 aa 1-18.
Sequence:MSATAATVPPAAPAGEGG
(Peptide available asab4958)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferConstituents: 3% Sodium deoxycholate, 3% Triton-X-100, 0.3% Tris HCl, 15% Sodium chloride
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
Applications
Our Abpromise guarantee covers the use of ab3347 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).Can be blocked with VAMP2 peptide (ab4958). | |
| IP | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | 1/1000. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 23056393 |
Target
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FunctionInvolved in the targeting and/or fusion of transport vesicles to their target membrane.
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Tissue specificityNervous system and skeletal muscle.
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Sequence similaritiesBelongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain. -
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
- Information by UniProt
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Database links
- Entrez Gene: 282116 Cow
- Entrez Gene: 6844 Human
- Entrez Gene: 22318 Mouse
- Entrez Gene: 24803 Rat
- Omim: 185881 Human
- SwissProt: P63027 Human
- SwissProt: P63044 Mouse
- SwissProt: P63045 Rat
see all -
Alternative names
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
see all
Images
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Western blot - Anti-VAMP2 antibody (ab3347)Western blot detection of VAMP2 on rat brain extract using ab3347.
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Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody (ab3347)This image is courtesy of an abreview submitted by Karine Thibault, Laboratoire de Neurobiologie, ParisVAMP2 antibody (ab3347) used in Immunohistochemistry (rat frozen sections). Sections were fixed with paraformaldehyde. Primary Antibody ab3347 used at 1/1000 incubated for 18 hours at 20°C in PBS-T 0.3%. Secondary Antibody used anti-IgG Rabbit Conjugation Alexa Fluor® 488 Dilution 1/1000. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-VAMP2 antibody (ab3347)Image from Zhang HH et al., PLoS One. 2012;7(10):e46666. doi: 10.1371/journal.pone.0046666. Epub 2012 Oct 2.Fig 2.; October 2, 2012 PLoS ONE 7(10): e46666.Immunohistochemical analysis of rat spinal cord dorsal horn tissue, staining VAMP2 with ab3347 at 1/200 dilution. Fluorescent-conjugated anti-rabbit IgG (1/200) was used as the secondary antibody.
Protocols
References
This product has been referenced in:
- Borgo C et al. CK2 modulates adipocyte insulin-signaling and is up-regulated in human obesity. Sci Rep 7:17569 (2017). Read more (PubMed: 29242563) »
- Gordon SL et al. A Fine Balance of Synaptophysin Levels Underlies Efficient Retrieval of Synaptobrevin II to Synaptic Vesicles. PLoS One 11:e0149457 (2016). Mouse . Read more (PubMed: 26871701) »
Customer reviews and Q&As
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