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    vamp2-antibody-ab3347.pdf

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Signal Transduction Protein Trafficking Vesicle Transport SNAPs & SNAREs
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Anti-VAMP2 antibody (ab3347)

  • Datasheet
  • SDS
Reviews (3)Q&A (2)References (37)

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Western blot - Anti-VAMP2 antibody (ab3347)

    Key features and details

    • Rabbit polyclonal to VAMP2
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

    You may also be interested in

    Secondary
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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
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    Recombinant Mouse VAMP2 protein (His tag) (ab222979)
    Primary
    Product image
    Anti-Synaptophysin antibody [YE269] (ab32127)

    View more associated products

    Overview

    • Product name

      Anti-VAMP2 antibody
      See all VAMP2 primary antibodies
    • Description

      Rabbit polyclonal to VAMP2
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Rat
      See all applications and species data
    • Immunogen

      Synthetic peptide corresponding to Rat VAMP2 aa 1-18.
      Sequence:

      MSATAATVPPAAPAGEGG


      (Peptide available as ab4958)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Constituents: PBS, 0.1% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Protein Trafficking
      • Vesicle Transport
      • SNAPs & SNAREs
      • Neuroscience
      • Cell Type Marker
      • Neuron marker
      • Synapse marker
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Integration of energy metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Integration of energy
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Mouse VAMP2 protein (His tag) (ab222979)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab3347 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Rat
    All applications
    Rhesus monkey
    Application Abreviews Notes
    WB (1)
    Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).
    Notes
    WB
    Use at an assay dependent concentration. Detects a band of approximately 19 kDa (predicted molecular weight: 19 kDa).

    Target

    • Function

      Involved in the targeting and/or fusion of transport vesicles to their target membrane.
    • Tissue specificity

      Nervous system and skeletal muscle.
    • Sequence similarities

      Belongs to the synaptobrevin family.
      Contains 1 v-SNARE coiled-coil homology domain.
    • Cellular localization

      Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
    • Target information above from: UniProt accession P63027 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 24803 Rat
      • SwissProt: P63045 Rat
      • Unigene: 12939 Rat
      • Alternative names

        • FLJ11460 antibody
        • RATVAMPB antibody
        • RATVAMPIR antibody
        • SYB antibody
        • SYB2 antibody
        • Synaptobrevin 2 antibody
        • Synaptobrevin-2 antibody
        • VAMP 2 antibody
        • VAMP-2 antibody
        • Vamp2 antibody
        • VAMP2_HUMAN antibody
        • Vesicle associated membrane protein 2 antibody
        • Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody
        • Vesicle-associated membrane protein 2 antibody
        see all

      Images

      • Western blot - Anti-VAMP2 antibody (ab3347)
        Western blot - Anti-VAMP2 antibody (ab3347)

        Western blot detection of VAMP2 on rat brain extract using ab3347.

      Protocols

      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (37)

      Publishing research using ab3347? Please let us know so that we can cite the reference in this datasheet.

      ab3347 has been referenced in 37 publications.

      • Kokotos AC  et al. Synaptophysin sustains presynaptic performance by preserving vesicular synaptobrevin-II levels. J Neurochem 151:28-37 (2019). PubMed: 31216055
      • Brandenburger T  et al. MiR-34a is differentially expressed in dorsal root ganglia in a rat model of chronic neuropathic pain. Neurosci Lett 708:134365 (2019). PubMed: 31278961
      • Joussain C  et al. Botulinum Neurotoxin Light Chains Expressed by Defective Herpes Simplex Virus Type-1 Vectors Cleave SNARE Proteins and Inhibit CGRP Release in Rat Sensory Neurons. Toxins (Basel) 11:N/A (2019). PubMed: 30791373
      • Hoffman BU  et al. Merkel Cells Activate Sensory Neural Pathways through Adrenergic Synapses. Neuron 100:1401-1413.e6 (2018). PubMed: 30415995
      • Actis Dato V  et al. Insulin-induced exocytosis regulates the cell surface level of low-density lipoprotein-related protein-1 in Müller Glial cells. Biochem J 475:1669-1685 (2018). PubMed: 29669912
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-5 of 5 Abreviews or Q&A

      Western blot abreview for Anti-VAMP2 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Loading amount
      2 µg
      Gel Running Conditions
      Non-reduced Denaturing
      Sample
      Rat Tissue lysate - whole (hippocampus tissue lysate)
      Specification
      hippocampus tissue lysate
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 27°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jul 07 2014

      Question

      In hopes of answering your questions:

      1) the problem is we are not seeing any bands even in a control sample prepared from kidney cortex.
      2) We are using protein samples from IMCD3 cell lystates. These were prepared using a RIPA lysis buffer.
      3) The primary antibody is being incubated overnight at 4 degrees Celsius.

      Read More

      Abcam community

      Verified customer

      Asked on Nov 05 2012

      Answer

      Thank you for your reply and the additional information.

      Please let me know your order or PO number. If the antibody was purchased within the last 6 months, our Abpromise guarantee applies and I'd be happy to offer you either a free of charge replacement, a credit or a refund.

      Please let me know which option would be best for you.

      I look forward to hear back and resolve this case for you.

      Read More

      Abcam Scientific Support

      Answered on Nov 05 2012

      Question

      We have attempted to use your Anti-VAMP2 antibody (ab3347) for Western Blotting. We have unfortunately had no success working with a 1:500 dilution. Is there a suggested protocol that you may be able to offer?

      Here are some highlights of our procedure:

      - 100ug protein loaded
      - 15 minute denaturation at 75 degrees Celsius
      - 1 Hour block with 10% Non Fat Dry Skim Milk (NFDSM)
      - 1:500 Anti-VAMP2 in 10% NFDSM
      - 90 min 1:2000 Secondary
      - 30 min ECL development

      Read More

      Abcam community

      Verified customer

      Asked on Nov 02 2012

      Answer

      Thank you for contacting us.
      I am sorry to hear that the antibody is not working as expected.

      I have copied the WB protocol below.
      Also,
      I have a few more questions in order to understand the issue better:
      1) What is the exact problem (e.g. no bands, high background)?
      2) What type of samples have you used and how were they prepared?
      3) How long did you incubate with the primary antibody?



      Western Blot Protocol

      Polyacrylamide gel electrophoresis and blottingAdd an appropriate amount of electrophoresis sample buffer (1X = 125mM Tris-HCl pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, and 1% beta-mercaptoethanol) to all samples.
      Heat to 95°C for 3-5 minutes.
      Load 5-100 ug total protein in a volume that is appropriate for the size of the wells.
      Electrophorese proteins for the appropriate time according to the manufacturers instructions.
      Transfer proteins from the gel to a suitable membrane (e.g. nitrocellulose, PVDF, etc.) following the manufacturers protocol for transfer.
      High molecular weight proteins (>120 kDa) can be wet transferred more efficiently if transfer time is increased and 0.05% SDS is included in the transfer buffer.


      BlockingRemove the filter from the transfer apparatus and rinse in PBST/TBST to remove loose acrylamide.
      Transferred proteins can be visualized by staining the membrane for 15-30 minutes with Ponceau S.
      Remove stain from filter by washing with PBST/TBST.
      Place filter into blocking solution.
      Block for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C.


      Incubation with primary antibodyDecant the blocking buffer and add the antibody, diluted in blocking buffer as suggested in the product description sheet.
      Incubate with agitation for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C.


      Incubation with secondary antibodyWash for 30 minutes with agitation in wash buffer (TBS or PBS with 0.1% Tween 20), changing the wash buffer every 5 minutes.
      Decant the wash solution and add AffiniClear HRP-conjugated secondary antibody, diluted in 5% non-fat dry milk in wash buffer.
      Incubate for 30 minutes at 37°C, 1 hour at room temperature, or overnight at 4°C.
      Decant the antibody conjugate and wash for 30 minutes with agitation in wash buffer (TBS or PBS with 0.1% Tween 20), changing the wash buffer every 5 minutes.


      Substrate incubation (ECL)Decant washing buffer and place the blot in a plastic bag or clean tray containing the development working solution (0.125 ml/cm2) for 1-5 minutes.
      Agitate bag or tray to cover the surface of the membrane.
      Remove the blot from the bag or tray and place it between two pieces of write-on transparency film.
      Smooth over the covered blot to remove air bubbles and excess substrate.
      Expose to X-ray film or any sensitive screen. (Check manufacturer's instructions for specific ECL reagents and procedures.)


      I look forward to hear back from you and assist you further.

      Read More

      Abcam Scientific Support

      Answered on Nov 02 2012

      Immunohistochemistry (Frozen sections) abreview for Anti-VAMP2 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Rat Tissue sections (Brain)
      Specification
      Brain
      Fixative
      Paraformaldehyde
      Permeabilization
      No
      Read More

      Dr. Karine Thibault

      Verified customer

      Submitted Apr 14 2009

      Immunocytochemistry/ Immunofluorescence abreview for Anti-VAMP2 antibody

      Good
      Abreviews
      Abreviews
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Rat Cell (hippocampal neuron)
      Specification
      hippocampal neuron
      Fixative
      Paraformaldehyde
      Blocking step
      BSA as blocking agent for 20 minute(s) · Concentration: 2%
      Read More

      Dr. Chanchoo Yap

      Verified customer

      Submitted Nov 27 2006

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