Recombinant
RabMAb

Recombinant Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

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Overview

  • Product name

    Anti-VAMP2 antibody [EPR12790] - BSA and Azide free
    See all VAMP2 primary antibodies

  • Description

    Rabbit monoclonal [EPR12790] to VAMP2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human VAMP2 aa 1-100. The exact sequence is proprietary.
    Database link: P63027

  • Positive control

    • WB: Human fetal brain and Human cerebellum lysates ICC/IF: SH-SY5Y cells. Flow cyt: Jurkat cells.

  • General notes

    Ab214590 is the carrier-free version of ab181869. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab214590 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab214590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).
IP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Involved in the targeting and/or fusion of transport vesicles to their target membrane.

  • Tissue specificity

    Nervous system and skeletal muscle.

  • Sequence similarities

    Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.

  • Cellular localization

    Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • FLJ11460 antibody
    • RATVAMPB antibody
    • RATVAMPIR antibody
    • SYB antibody
    • SYB2 antibody
    • Synaptobrevin 2 antibody
    • Synaptobrevin-2 antibody
    • VAMP 2 antibody
    • VAMP-2 antibody
    • Vamp2 antibody
    • VAMP2_HUMAN antibody
    • Vesicle associated membrane protein 2 antibody
    • Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody
    • Vesicle-associated membrane protein 2 antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    ab181869 staining VAMP2 in U87-MG (human glioblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1:PBS only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    ab181869 (purified) at 1/150 immunoprecipitating VAMP2 in Human cerebellum whole cell lysate. 10 ug of cell lysate was present in the input. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Flow Cytometry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Flow Cytometry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    Flow Cytometry analysis of SH-SY5Y cells labelling VAMP2 with purified ab181869 at 1/100 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    Immunocytochemistry/Immunofluorescence analysis of U87-MG (human glioblastoma) cells labelling VAMP2 with purified ab181869 at 1/250. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    Western blot analysis of immunoprecipitation pellet from Human cerebellum lysate immunoprecipitated using ab181869 at 1/70 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Flow Cytometry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Flow Cytometry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    Flow cytometry analysis of Jurkat cells fixed with 2% paraformaldehyde labeling VAMP2 with unpurified ab181869 at 1/150 dilution followed by Goat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    Immunofluorescent analysis of SH-SYSY cells (4% paraformaldehyde-fixed) labeling VAMP2 with unpurified ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution and counterstained with DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Immunocytochemistry/ Immunofluorescence - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    Immunofluorescent analysis of U87-MG cells (4% paraformaldehyde-fixed)  labeling VAMP2 with ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution and counterstained with Dapi.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

References

This product has been referenced in:

  • Xu S  et al. Autocrine insulin increases plasma membrane KATP channel via PI3K-VAMP2 pathway in MIN6 cells. Biochem Biophys Res Commun 468:752-7 (2015). WB ; Mouse . Read more (PubMed: 26585489) »
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