For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

We use cookies to make our site as useful as possible.

Our Cookie Policy explains how you can opt-out of the cookies we use.

If you continue without changing your cookie settings, we'll assume you’re happy with this.

Continue Continue

United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Diagnostic & Therapeutic Solutions
    Custom solutions & partnerships

    Custom antibody development and commercial partnerships to advance your diagnostic and therapeutic discovery.

    Create custom solutions with us

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Supporting our customers and employees during the COVID-19 pandemic. Read more

  1. Link

    vamp2-antibody-epr12790-bsa-and-azide-free-ab214590.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Signal Transduction Protein Trafficking Vesicle Transport SNAPs & SNAREs
Share by email
RecombinantRabMAb

Recombinant Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (1)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
  • Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
  • Flow Cytometry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
  • Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
  • Flow Cytometry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
  • Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
  • Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
  • Flow Cytometry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
  • Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
  • Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
  • Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12790] to VAMP2 - BSA and Azide free
  • Suitable for: ICC, WB, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Protein
Product image
Recombinant Mouse VAMP2 protein (His tag) (ab222979)
Conjugation
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)

View more associated products

Overview

  • Product name

    Anti-VAMP2 antibody [EPR12790] - BSA and Azide free
    See all VAMP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR12790] to VAMP2 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC, WB, IP, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VAMP2 aa 1-100. The exact sequence is proprietary.
    Database link: P63027

  • Positive control

    • WB: Human fetal brain and Human cerebellum lysates ICC/IF: SH-SY5Y cells. Flow cyt: Jurkat cells.
  • General notes

    Ab214590 is the carrier-free version of ab181869. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab214590 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR12790
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • SNAPs & SNAREs
    • Neuroscience
    • Cell Type Marker
    • Neuron marker
    • Synapse marker
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy

Associated products

  • Alternative Versions

    • Anti-VAMP2 antibody [EPR12790] (ab181869)
    • HRP Anti-VAMP2 antibody [EPR12790] (ab198948)
    • Alexa Fluor® 647 Anti-VAMP2 antibody [EPR12790] (ab198949)
    • PE Anti-VAMP2 antibody [EPR12790] (ab214529)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Mouse VAMP2 protein (His tag) (ab222979)

Applications

Our Abpromise guarantee covers the use of ab214590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).
IP Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Involved in the targeting and/or fusion of transport vesicles to their target membrane.
  • Tissue specificity

    Nervous system and skeletal muscle.
  • Sequence similarities

    Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localization

    Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
  • Target information above from: UniProt accession P63027 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6844 Human
    • Entrez Gene: 22318 Mouse
    • Entrez Gene: 24803 Rat
    • Omim: 185881 Human
    • SwissProt: P63027 Human
    • SwissProt: P63044 Mouse
    • SwissProt: P63045 Rat
    • Unigene: 25348 Human
    • Unigene: 28643 Mouse
    • Unigene: 12939 Rat
    see all
  • Alternative names

    • FLJ11460 antibody
    • RATVAMPB antibody
    • RATVAMPIR antibody
    • SYB antibody
    • SYB2 antibody
    • Synaptobrevin 2 antibody
    • Synaptobrevin-2 antibody
    • VAMP 2 antibody
    • VAMP-2 antibody
    • Vamp2 antibody
    • VAMP2_HUMAN antibody
    • Vesicle associated membrane protein 2 antibody
    • Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody
    • Vesicle-associated membrane protein 2 antibody
    see all

Images

  • Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    ab181869 staining VAMP2 in U87-MG (human glioblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.

    Negative control 1:PBS only.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    ab181869 (purified) at 1/150 immunoprecipitating VAMP2 in Human cerebellum whole cell lysate. 10 ug of cell lysate was present in the input. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Flow Cytometry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Flow Cytometry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    Clone EPR12790 (ab214590) has been successfully conjugated by Abcam. This image was generated using Anti-VAMP2 antibody [EPR12790] (PE). Please refer to ab214529 for protocol details.

    Overlay histogram showing U-87MG cells stained with ab214529 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab214529, 1/2500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in U-87MG cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

  • Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    Clone EPR12790 (ab214590) has been successfully conjugated by Abcam. This image was generated using Anti-VAMP2 antibody [EPR12790] (Alexa Fluor® 647). Please refer to ab198949 for protocol details.

    ab198949 staining VAMP2 in U87MG cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198949 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Flow Cytometry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    Flow Cytometry analysis of SH-SY5Y cells labelling VAMP2 with purified ab181869 at 1/100 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    Immunocytochemistry/Immunofluorescence analysis of U87-MG (human glioblastoma) cells labelling VAMP2 with purified ab181869 at 1/250. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Immunoprecipitation - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    Western blot analysis of immunoprecipitation pellet from Human cerebellum lysate immunoprecipitated using ab181869 at 1/70 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Flow Cytometry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Flow Cytometry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    Flow cytometry analysis of Jurkat cells fixed with 2% paraformaldehyde labeling VAMP2 with unpurified ab181869 at 1/150 dilution followed by Goat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    Immunofluorescent analysis of SH-SYSY cells (4% paraformaldehyde-fixed) labeling VAMP2 with unpurified ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution and counterstained with DAPI.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Immunocytochemistry - Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

    Immunofluorescent analysis of U87-MG cells (4% paraformaldehyde-fixed)  labeling VAMP2 with ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution and counterstained with Dapi.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).

  • Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
    Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (1)

    Publishing research using ab214590? Please let us know so that we can cite the reference in this datasheet.

    ab214590 has been referenced in 1 publication.

    • Xu S  et al. Autocrine insulin increases plasma membrane KATP channel via PI3K-VAMP2 pathway in MIN6 cells. Biochem Biophys Res Commun 468:752-7 (2015). WB ; Mouse . PubMed: 26585489

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    There are currently no Customer reviews or Questions for ab214590.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Get resources and offers direct to your inbox Sign up
    A-Z by research area
    • Cancer
    • Cardiovascular
    • Cell biology
    • Developmental biology
    • Epigenetics & Nuclear signaling
    • Immunology
    • Metabolism
    • Microbiology
    • Neuroscience
    • Signal transduction
    • Stem cells
    A-Z by product type
    • Primary antibodies
    • Secondary antibodies
    • Biochemicals
    • Isotype controls
    • Flow cytometry multi-color selector
    • Kits
    • Loading controls
    • Lysates
    • Peptides
    • Proteins
    • Slides
    • Tags and cell markers
    • Tools & Reagents
    Help & support
    • Support
    • Make an Inquiry
    • Protocols & troubleshooting
    • Placing an order
    • RabMAb products
    • Biochemical product FAQs
    • Training
    • Browse by Target
    Company
    • Corporate site
    • Investor relations
    • Company news
    • Careers
    • About us
    • Blog
    Events
    • Tradeshows
    • Conferences
    International websites
    • abcam.cn
    • abcam.co.jp

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    • Modern slavery statement
    © 1998-2021 Abcam plc. All rights reserved.