Recombinant Anti-VAMP2 antibody [EPR12790] - BSA and Azide free (ab214590)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12790] to VAMP2 - BSA and Azide free
- Suitable for: ICC, WB, IP, Flow Cyt
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-VAMP2 antibody [EPR12790] - BSA and Azide free
See all VAMP2 primary antibodies -
Description
Rabbit monoclonal [EPR12790] to VAMP2 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: ICC, WB, IP, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human VAMP2 aa 1-100. The exact sequence is proprietary.
Database link: P63027 -
Positive control
- WB: Human fetal brain and Human cerebellum lysates ICC/IF: SH-SY5Y cells. Flow cyt: Jurkat cells.
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General notes
Ab214590 is the carrier-free version of ab181869. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab214590 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR12790 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab214590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC | Use at an assay dependent concentration. | |
WB | Use at an assay dependent concentration. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa). | |
IP | Use at an assay dependent concentration. | |
Flow Cyt | Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Target
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Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane. -
Tissue specificity
Nervous system and skeletal muscle. -
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles. - Information by UniProt
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Database links
- Entrez Gene: 6844 Human
- Entrez Gene: 22318 Mouse
- Entrez Gene: 24803 Rat
- Omim: 185881 Human
- SwissProt: P63027 Human
- SwissProt: P63044 Mouse
- SwissProt: P63045 Rat
- Unigene: 25348 Human
see all -
Alternative names
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
see all
Images
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ab181869 staining VAMP2 in U87-MG (human glioblastoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. DAPI was used as a nuclear counterstain.
Negative control 1:PBS only.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).
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ab181869 (purified) at 1/150 immunoprecipitating VAMP2 in Human cerebellum whole cell lysate. 10 ug of cell lysate was present in the input. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).
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Clone EPR12790 (ab214590) has been successfully conjugated by Abcam. This image was generated using Anti-VAMP2 antibody [EPR12790] (PE). Please refer to ab214529 for protocol details.
Overlay histogram showing U-87MG cells stained with ab214529 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab214529, 1/2500 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was Rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
This antibody gave a positive signal in U-87MG cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
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Clone EPR12790 (ab214590) has been successfully conjugated by Abcam. This image was generated using Anti-VAMP2 antibody [EPR12790] (Alexa Fluor® 647). Please refer to ab198949 for protocol details.
ab198949 staining VAMP2 in U87MG cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab198949 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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Flow Cytometry analysis of SH-SY5Y cells labelling VAMP2 with purified ab181869 at 1/100 (red). Cells were fixed with 4% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).
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Immunocytochemistry/Immunofluorescence analysis of U87-MG (human glioblastoma) cells labelling VAMP2 with purified ab181869 at 1/250. Cells were fixed with 100% methanol and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).
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Western blot analysis of immunoprecipitation pellet from Human cerebellum lysate immunoprecipitated using ab181869 at 1/70 dilution.
Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).
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Flow cytometry analysis of Jurkat cells fixed with 2% paraformaldehyde labeling VAMP2 with unpurified ab181869 at 1/150 dilution followed by Goat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).
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Immunofluorescent analysis of SH-SYSY cells (4% paraformaldehyde-fixed) labeling VAMP2 with unpurified ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution and counterstained with DAPI.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).
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Immunofluorescent analysis of U87-MG cells (4% paraformaldehyde-fixed) labeling VAMP2 with ab181869 at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution and counterstained with Dapi.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181869).
Protocols
Datasheets and documents
Certificate of Compliance
References (1)
ab214590 has been referenced in 1 publication.
- Xu S et al. Autocrine insulin increases plasma membrane KATP channel via PI3K-VAMP2 pathway in MIN6 cells. Biochem Biophys Res Commun 468:752-7 (2015). WB ; Mouse . PubMed: 26585489