Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12790] to VAMP2 (HRP)
- Suitable for: WB
- Reacts with: Human
- Conjugation: HRP
Product nameAnti-VAMP2 antibody [EPR12790] (HRP)
See all VAMP2 primary antibodies
DescriptionRabbit monoclonal [EPR12790] to VAMP2 (HRP)
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human VAMP2 aa 1-100. The exact sequence is proprietary.
Database link: P63027
- WB: Human fetal brain lysate.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: PBS, 1% BSA, 30% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab198948 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000. Detects a band of approximately 18 kDa (predicted molecular weight: 13 kDa).|
FunctionInvolved in the targeting and/or fusion of transport vesicles to their target membrane.
Tissue specificityNervous system and skeletal muscle.
Sequence similaritiesBelongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
- Information by UniProt
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
Anti-VAMP2 antibody [EPR12790] (HRP) (ab198948) at 1/5000 dilution + Brain (Human) Tissue Lysate - fetal normal tissue at 10 µg
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 13 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Exposure time: 10 seconds
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab198948 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab198948 has not yet been referenced specifically in any publications.