Overview

  • Product name

    Anti-VAMP2 antibody [EPR20818]
    See all VAMP2 primary antibodies
  • Description

    Rabbit monoclonal [EPR20818] to VAMP2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VAMP2 aa 1-100. The exact sequence is proprietary.
    Database link: P63044

  • Positive control

    • WB: Neuro-2a, U-87 MG, SH-SY5Y, NIH/3T3, RAW 264.7, PC-12 and C6 whole cell lysates; Human cerebellum and fetal brain lysates; Mouse brain, hippocampus and cerebellum lysates; Rat heart, hippocampus and cerebellum lysates. IHC-P: Human kidney tissue; Mouse and rat cerebrum tissues. IP: Mouse brain lysate.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab215721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 12 kDa).

Target

  • Function

    Involved in the targeting and/or fusion of transport vesicles to their target membrane.
  • Tissue specificity

    Nervous system and skeletal muscle.
  • Sequence similarities

    Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localization

    Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ11460 antibody
    • RATVAMPB antibody
    • RATVAMPIR antibody
    • SYB antibody
    • SYB2 antibody
    • Synaptobrevin 2 antibody
    • Synaptobrevin-2 antibody
    • VAMP 2 antibody
    • VAMP-2 antibody
    • Vamp2 antibody
    • VAMP2_HUMAN antibody
    • Vesicle associated membrane protein 2 antibody
    • Vesicle-associated membrane protein 2 (synaptobrevin 2) antibody
    • Vesicle-associated membrane protein 2 antibody
    see all

Images

  • All lanes : Anti-VAMP2 antibody [EPR20818] (ab215721) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse hippocampus lysate
    Lane 3 : Mouse cerebellum lysate
    Lane 4 : Neuro-2a (mouse neuroblastoma cell line), whole cell lysate
    Lane 5 : PC-12 (rat adrenal gland pheochromocytoma cell line), whole cell lysate
    Lane 6 : C6 (rat glial tumor cell line), whole cell lysate
    Lane 7 : Rat cerebellum lysate
    Lane 8 : Rat hippocampus lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 12 kDa
    Observed band size: 18 kDa
    why is the actual band size different from the predicted?



     

    Exposure times: Lanes 1-3, 7-8: 1 second; Lanes 4-5: 3 minutes.

    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human kidney. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

  • VAMP2 was immunoprecipitated from 0.35 mg mouse brain lysate with ab215721 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215721 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution

    Lane 1: Mouse brain lysate 10 μg (Input).

    Lane 2: ab215721 IP in mouse brain lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of  ab215721 in mouse brain lysate.

    Exposure time: 1 second.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

  • All lanes : Anti-VAMP2 antibody [EPR20818] (ab215721) at 1/1000 dilution

    Lane 1 : Rat heart lysate
    Lane 2 : Human fetal brain lysate
    Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line), whole cell lysate
    Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow), whole cell lysate
    Lane 5 : NIH/3T3 (mouse embryonic fibroblast cell line), whole cell lysate
    Lane 6 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus), whole cell lysate
    Lane 7 : Human cerebellum lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 12 kDa
    Observed band size: 18 kDa why is the actual band size different from the predicted?



     

    Exposure times: Lanes 1,3-6: 3 minutes; Lanes 2,7: 1 second.

    Blocking/Dilution buffer: 5% NFDM/TBST.

     

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse cerebrum. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

  • Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on rat cerebrum. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

References

ab215721 has not yet been referenced specifically in any publications.

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