Recombinant Anti-VAMP2 antibody [EPR20818] (ab215721)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20818] to VAMP2
- Suitable for: IP, IHC-P, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-VAMP2 antibody [EPR20818]
See all VAMP2 primary antibodies -
Description
Rabbit monoclonal [EPR20818] to VAMP2 -
Host species
Rabbit -
Tested applications
Suitable for: IP, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Neuro-2a, U-87 MG, SH-SY5Y, NIH/3T3, RAW 264.7, PC-12 and C6 whole cell lysates; Human cerebellum and fetal brain lysates; Mouse brain, hippocampus and cerebellum lysates; Rat heart, hippocampus and cerebellum lysates. IHC-P: Human kidney tissue; Mouse and rat cerebrum tissues. IP: Mouse brain lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20818 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab215721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
1/30.
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IHC-P | (3) |
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
WB |
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 12 kDa).
|
Notes |
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IP
1/30. |
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Detects a band of approximately 18 kDa (predicted molecular weight: 12 kDa). |
Target
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Function
Involved in the targeting and/or fusion of transport vesicles to their target membrane. -
Tissue specificity
Nervous system and skeletal muscle. -
Sequence similarities
Belongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain. -
Cellular localization
Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome. Neuronal synaptic vesicles. - Information by UniProt
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Database links
- Entrez Gene: 6844 Human
- Entrez Gene: 22318 Mouse
- Entrez Gene: 24803 Rat
- Omim: 185881 Human
- SwissProt: P63027 Human
- SwissProt: P63044 Mouse
- SwissProt: P63045 Rat
- Unigene: 25348 Human
see all -
Alternative names
- FLJ11460 antibody
- RATVAMPB antibody
- RATVAMPIR antibody
see all
Images
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All lanes : Anti-VAMP2 antibody [EPR20818] (ab215721) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse hippocampus lysate
Lane 3 : Mouse cerebellum lysate
Lane 4 : Neuro-2a (mouse neuroblastoma cell line), whole cell lysate
Lane 5 : PC-12 (rat adrenal gland pheochromocytoma cell line), whole cell lysate
Lane 6 : C6 (rat glial tumor cell line), whole cell lysate
Lane 7 : Rat cerebellum lysate
Lane 8 : Rat hippocampus lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1-3, 7-8: 1 second; Lanes 4-5: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on human kidney. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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VAMP2 was immunoprecipitated from 0.35 mg mouse brain lysate with ab215721 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab215721 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution
Lane 1: Mouse brain lysate 10 μg (Input).
Lane 2: ab215721 IP in mouse brain lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab215721 in mouse brain lysate.
Exposure time: 1 second.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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All lanes : Anti-VAMP2 antibody [EPR20818] (ab215721) at 1/1000 dilution
Lane 1 : Rat heart lysate
Lane 2 : Human fetal brain lysate
Lane 3 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line), whole cell lysate
Lane 4 : SH-SY5Y (human neuroblastoma cell line from bone marrow), whole cell lysate
Lane 5 : NIH/3T3 (mouse embryonic fibroblast cell line), whole cell lysate
Lane 6 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus), whole cell lysate
Lane 7 : Human cerebellum lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 12 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?Exposure times: Lanes 1,3-6: 3 minutes; Lanes 2,7: 1 second.
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on mouse cerebrum. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling VAMP2 with ab215721 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Cytoplasmic staining on rat cerebrum. Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab215721 has been referenced in 1 publication.
- Asadi F & Dhanvantari S Stathmin-2 Mediates Glucagon Secretion From Pancreatic a-Cells. Front Endocrinol (Lausanne) 11:29 (2020). PubMed: 32117057