Key features and details
- Rabbit polyclonal to VAMP4
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-VAMP4 antibody
See all VAMP4 primary antibodies
DescriptionRabbit polyclonal to VAMP4
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
- IHC-P: Human cerebral cortex, cerebellum, endometrium and skeletal muscle tissues. ICC/IF: A431 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab251938 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
FunctionInvolved in the pathway that functions to remove an inhibitor (probably synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. May be a marker for this sorting pathway that is critical for remodeling the secretory response of granule.
Sequence similaritiesBelongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
Cellular localizationGolgi apparatus > trans-Golgi network membrane. Associated with trans Golgi network (TGN) and newly formed immature secretory granules (ISG). Not found on the mature secretory organelles.
- Information by UniProt
- VAMP 24 antibody
- VAMP 4 antibody
- VAMP-4 antibody
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for VAMP4 (green) using ab251938 at 4 µg/ml in ICC/IF.
Paraffin-embedded human cerebral cortex tissue stained for VAMP4 using ab251938 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human endometrium tissue stained for VAMP4 using ab251938 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human cerebellum tissue stained for VAMP4 using ab251938 at 1/200 dilution in immunohistochemical analysis.
Paraffin-embedded human skeletal muscle tissue stained for VAMP4 using ab251938 at 1/200 dilution in immunohistochemical analysis. Weak positivity.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab251938 has not yet been referenced specifically in any publications.