Product nameAnti-VAMP8 / EDB antibody
See all VAMP8 / EDB primary antibodies
DescriptionRabbit polyclonal to VAMP8 / EDB
SpecificityReacts specifically with the 10 kDa protein of human CaCo-2, rat PC12 and dog MDCK cell extracts.
Tested applicationsSuitable for: WB, IP, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Dog
Synthetic peptide corresponding to Human VAMP8/ EDB.
- human CaCo-2, rat PC12 and dog MDCK cell extracts. This antibody also gave a positive signal in the following Human whole cell lysates: A431; HEK293; HeLa.
Previously labelled as VAMP8.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferConstituent: Whole serum
Our Abpromise guarantee covers the use of ab6186 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IM: Use at an assay dependant dilution.
IP: Use at an assay dependant dilution.
WB: 1/200 - 1/1000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionInvolved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.
Sequence similaritiesBelongs to the synaptobrevin family.
Contains 1 v-SNARE coiled-coil homology domain.
- Information by UniProt
- EDB antibody
- Endobrevin antibody
- VAMP 8 antibody
All lanes : Anti-VAMP8 / EDB antibody (ab6186) at 1 µg/ml
Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 11 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
This product has been referenced in:
- Yang Y et al. Polyphenols differentially inhibit degranulation of distinct subsets of vesicles in mast cells by specific interaction with granule-type-dependent SNARE complexes. Biochem J 450:537-46 (2013). Read more (PubMed: 23252429) »
- Bethani I et al. Endosomal fusion upon SNARE knockdown is maintained by residual SNARE activity and enhanced docking. Traffic 10:1543-59 (2009). WB . Read more (PubMed: 19624487) »