Product nameVAMP8 / EDB overexpression 293T lysate (whole cell)
See all VAMP8/EDB lysates
ab94103 is a 293T cell transfected lysate in which Human VAMP8 / EDB has been transiently over-expressed using a pCMV-VAMP8 / EDB plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
Tested applicationsSuitable for: WBmore details
Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
Storage bufferConstituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine)
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BackgroundFunction: Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes. Tissue specificity: Platelets. Similarity: Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.
Our Abpromise guarantee covers the use of ab94103 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent dilution.|
ab94103 at 15µg/lane on an SDS-PAGE gel.
All lanes : Anti-VAMP8/EDB antibody (ab89158) at 1/500 dilution
Lane 1 :
VAMP8 / EDB overexpression 293T lysate (whole cell) (ab94103)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
ab94103 has not yet been referenced specifically in any publications.