Recombinant
RabMAb

Recombinant Anti-VAMP8/EDB antibody [EP2629Y] (Phycoerythrin) (ab208831)

Overview

  • Product name

    Anti-VAMP8/EDB antibody [EP2629Y] (Phycoerythrin)
    See all VAMP8/EDB primary antibodies
  • Description

    Rabbit monoclonal [EP2629Y] to VAMP8/EDB (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human VAMP8/EDB aa 1 to the C-terminus (N terminal). The exact sequence is proprietary.
    Database link: Q9BV40

  • Positive control

    • Flow Cyt: HeLa cells.
  • General notes

    Previously labelled as VAMP8. 

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab208831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/2500.

The cellular localisation of this product has been verified in ICC/IF

Target

  • Function

    Involved in the targeting and/or fusion of transport vesicles to their target membrane. Involved for dense-granule secretion in platelets. Plays a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.
  • Tissue specificity

    Platelets.
  • Sequence similarities

    Belongs to the synaptobrevin family.
    Contains 1 v-SNARE coiled-coil homology domain.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • EDB antibody
    • Endobrevin antibody
    • VAMP 8 antibody
    • VAMP-8 antibody
    • VAMP8 antibody
    • VAMP8_HUMAN antibody
    • Vesicle associated membrane protein 8 antibody
    • Vesicle-associated membrane protein 8 antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab208831 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab208831, 1/2500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab208831 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab208831.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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