Overview

  • Product name
    Anti-VANGL1 antibody [EPR12415]
    See all VANGL1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12415] to VANGL1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VANGL1 aa 200-300 (internal sequence) (Cysteine residue). The exact sequence is proprietary.
    Database link: Q8TAA9

  • Positive control
    • MCF7, HeLa, Jurkat and MOLT4 cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR12415
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab176575 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 60 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Tissue specificity
      Accordiing to PubMed:11956595, ubiquitously expressed. According to PubMed:12011995, expressed specifically in testis and ovary.
    • Involvement in disease
      Defects in VANGL1 are a cause of neural tube defects (NTD) [MIM:182940]. NTD are congenital malformations. The most common forms of NTD are described as open defects (including anencephaly and myelomeningocele, or spina bifida), which result from the failure of fusion in the cranial and spinal region of the neural tube, respectively. Other open dysraphisms (including myeloschisis, hemimyelomeningocele, and hemimyelocele) are sometimes associated with a Chiari type 2 malformation. A number of skin-covered (closed) NTD are categorized clinically depending on the presence of a subcutaneous mass (lipomyeloschisis, lipomyelomeningocele, meningocele, and myelocystocele) or the absence of such a mass (complex dysraphic states, including split cord malformations, dermal sinus, caudal regression, and segmental spinal dysgenesis).
      Defects in VANGL1 are a cause of sacral defect with anterior meningocele (SDAM) [MIM:600145]. SDAM is a form of caudal dysgenesis. It is present at birth and becomes symptomatic later in life, usually because of obstructive labor in females, chronic constipation, or meningitis. Inheritance is autosomal dominant.
    • Sequence similarities
      Belongs to the Vang family.
    • Cellular localization
      Membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • ANGL planar cell polarity protein 1 antibody
      • KAI1 C-terminal interacting tetraspanin antibody
      • KITENIN antibody
      • Loop tail protein 2 homolog antibody
      • Loop-tail protein 2 homolog antibody
      • LPP2 antibody
      • MGC5338 antibody
      • STB2 antibody
      • STBM2 antibody
      • Strabismus 2 antibody
      • Strabismus, Drosophila, homolog of, 2 antibody
      • Van Gogh like protein 1 antibody
      • Van Gogh, Drosophila, homolog of, 1 antibody
      • Van Gogh-like protein 1 antibody
      • Vang like 1 (van gogh, Drosophila) antibody
      • Vang like protein 1 antibody
      • Vang-like 1 antibody
      • Vang-like protein 1 antibody
      • VANG1_HUMAN antibody
      • VANGL 1 antibody
      • Vangl1 antibody
      see all

    Images

    • All lanes : Anti-VANGL1 antibody [EPR12415] (ab176575) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : MOLT4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Developed using the ECL technique.

      Predicted band size: 60 kDa

    References

    ab176575 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Sample
    Mouse Tissue sections (Female reproductive - Ovary/Oviduct/Uterus)
    Specification
    Female reproductive - Ovary/Oviduct/Uterus
    Permeabilization
    Yes - PBST (PBS/0.1% v/v TritonX100 (sigma))
    Fixative
    TCA

    Abcam user community

    Verified customer

    Submitted Nov 24 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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