Key features and details
- Goat polyclonal to VANGL2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-VANGL2 antibody
See all VANGL2 primary antibodies
DescriptionGoat polyclonal to VANGL2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Xenopus laevis, Zebrafish
- WB: Mouse brain tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab60172 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa).
1 hour primary incubation is recommended for this product.
FunctionInvolved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. Plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. Required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process.
Sequence similaritiesBelongs to the Vang family.
- Information by UniProt
- Homolog of Drosophila strabismus antibody
- KIAA1215 antibody
- Loop tail associated protein antibody
Anti-VANGL2 antibody (ab60172) at 0.3 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer)
Predicted band size: 60 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
ab60172 has been referenced in 1 publication.
- Li H et al. miR-542-3p overexpression is associated with enhanced osteosarcoma cell proliferation and migration ability by targeting Van Gogh-like 2. Mol Med Rep 11:851-6 (2015). WB ; Human . PubMed: 25352048