Overview

  • Product name

  • Description

    Goat polyclonal to VANGL2
  • Host species

    Goat
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    C-QKYLRTTKQQPYH

    , corresponding to internal sequence amino acids 403-415 of Human VANGL2

  • Positive control

    • WB: Mouse brain tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab60172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 60 kDa).

Target

  • Function

    Involved in the control of early morphogenesis and patterning of both axial midline structures and the development of neural plate. Plays a role in the regulation of planar cell polarity, particularly in the orientation of stereociliary bundles in the cochlea. Required for polarization and movement of myocardializing cells in the outflow tract and seems to act via RHOA signaling to regulate this process.
  • Sequence similarities

    Belongs to the Vang family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Homolog of Drosophila strabismus antibody
    • KIAA1215 antibody
    • Loop tail associated protein antibody
    • loop tail protein 1 homolog antibody
    • Loop-tail protein 1 homolog antibody
    • LPP 1 antibody
    • LPP1 antibody
    • LTAP antibody
    • MGC119403 antibody
    • MGC119404 antibody
    • STB 1 antibody
    • STB1 antibody
    • STBM 1 antibody
    • STBM antibody
    • STBM1 antibody
    • Strabismus 1 antibody
    • van Gogh like protein 2 antibody
    • Van Gogh-like protein 2 antibody
    • Vang (van gogh, Drosophila) like 2 antibody
    • Vang like 2 (van gogh, Drosophila) antibody
    • vang like 2 antibody
    • vang like protein 2 antibody
    • Vang-like protein 2 antibody
    • VANG2_HUMAN antibody
    • VANGL 2 antibody
    • VANGL planar cell polarity protein 2 antibody
    • Vangl2 antibody
    see all

Images

  • Anti-VANGL2 antibody (ab60172) at 0.3 µg/ml + Mouse Brain lysate (35µg protein in RIPA buffer)

    Predicted band size: 60 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    Primary incubation was 1 hour. Detected by chemiluminescence.

References

This product has been referenced in:

  • Li H  et al. miR-542-3p overexpression is associated with enhanced osteosarcoma cell proliferation and migration ability by targeting Van Gogh-like 2. Mol Med Rep 11:851-6 (2015). WB ; Human . Read more (PubMed: 25352048) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Sample
Zebrafish Tissue lysate - whole (whole embryo)
Specification
whole embryo
Blocking step
BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Feb 05 2015

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Sample
Mouse Tissue sections (Adult Female reproductive - Ovary/Oviduct/Uterus)
Specification
Adult Female reproductive - Ovary/Oviduct/Uterus
Permeabilization
Yes - PBST (PBS/0.1% v/v TritonX100 (sigma))
Fixative
TCA

Dr. Julia Watson

Verified customer

Submitted Nov 24 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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