Overview

  • Product name

  • Description

    Rabbit polyclonal to VAP1
  • Host species

    Rabbit
  • Specificity

    This antibdoy detects endogenous level of total VAP1 protein.
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment within Human VAP1 (N terminal). The exact sequence is proprietary.
    Database link: Q16853

  • Positive control

    • Mouse kidney, liver and heart tissue lysates; A549 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab196739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 84 kDa.
IHC-P 1/50 - 1/200.
ICC/IF 1/50 - 1/200.

Target

  • Function

    Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.
  • Tissue specificity

    Strongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis.
  • Sequence similarities

    Belongs to the copper/topaquinone oxidase family.
  • Post-translational
    modifications

    Topaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
    N- and O-glycosylated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Amine oxidase, copper containing 3 (vascular adhesion protein 1) antibody
    • AOC3 antibody
    • AOC3_HUMAN antibody
    • Copper amine oxidase antibody
    • HPAO antibody
    • Membrane copper amine oxidase antibody
    • Membrane primary amine oxidase antibody
    • Semicarbazide sensitive amine oxidase antibody
    • Semicarbazide-sensitive amine oxidase antibody
    • SSAO antibody
    • VAP-1 antibody
    • Vascular adhesion protein 1 antibody
    • VP97 antibody
    see all

Images

  • All lanes : Anti-VAP1 antibody (ab196739) at 1/500 dilution

    Lane 1 : mouse kidney tissue lysate
    Lane 2 : mouse liver tissue lysate
    Lane 3 : mouse heart tissue lysate

    Predicted band size: 84 kDa

  • Immunofluorescent analysis of A549 cells labeling VAP1 using ab196739 at 1/50 dilution.

    Blue: DAPI for nuclear staining.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Specification
colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde

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Verified customer

Submitted Jun 11 2019

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