Key features and details
- Rabbit polyclonal to VAP1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-VAP1 antibody
See all VAP1 primary antibodies
DescriptionRabbit polyclonal to VAP1
SpecificityThis antibdoy detects endogenous level of total VAP1 protein.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment within Human VAP1 (N terminal). The exact sequence is proprietary.
Database link: Q16853
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.87% Sodium chloride
without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 84 kDa.|
|ICC/IF||1/50 - 1/200.|
FunctionCell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.
Tissue specificityStrongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis.
Sequence similaritiesBelongs to the copper/topaquinone oxidase family.
modificationsTopaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
N- and O-glycosylated.
- Information by UniProt
- Amine oxidase, copper containing 3 (vascular adhesion protein 1) antibody
- AOC3 antibody
- AOC3_HUMAN antibody
All lanes : Anti-VAP1 antibody (ab196739) at 1/500 dilution
Lane 1 : mouse kidney tissue lysate
Lane 2 : mouse liver tissue lysate
Lane 3 : mouse heart tissue lysate
Predicted band size: 84 kDa
Immunofluorescent analysis of A549 cells labeling VAP1 using ab196739 at 1/50 dilution.
Blue: DAPI for nuclear staining.
ab196739 has been referenced in 1 publication.
- Abu El-Asrar AM et al. Association of HMGB1 with oxidative stress markers and regulators in PDR. Mol Vis 23:853-871 (2017). PubMed: 29259392