Product nameAnti-VAP1 antibody
See all VAP1 primary antibodies
DescriptionRabbit polyclonal to VAP1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- This antibody gave a positive signal in the following Tissue Lysates: Lung (Rat), Lung (Mouse) and Lung (Human). This antibody gave a positive result in IHC in the following FFPE tissue: Human normal lung.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab42885 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 85 kDa).|
FunctionCell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin-independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis.
Tissue specificityStrongly expressed on the high endothelial venules of peripheral lymph nodes and on hepatic endothelia. Also highly expressed in appendix, lung and small intestine. Expressed also in adipose tissue, in bone marrow, colon, heart, kidney, ovary, pancreas, placenta, prostate, skeletal muscle, spleen and testis.
Sequence similaritiesBelongs to the copper/topaquinone oxidase family.
modificationsTopaquinone (TPQ) is generated by copper-dependent autoxidation of a specific tyrosyl residue.
N- and O-glycosylated.
- Information by UniProt
- Amine oxidase, copper containing 3 (vascular adhesion protein 1) antibody
- AOC3 antibody
- AOC3_HUMAN antibody
IHC image of VAP1 staining in Human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab42885, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-VAP1 antibody (ab42885) at 1 µg/ml
Lane 1 : Lung (Rat) Tissue Lysate
Lane 2 : Lung (Mouse) Whole Cell Lysate - normal tissue
Lane 3 : Lung (Human) Tissue Lysate - Fetal Normal Tissue
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 85 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa. We are unsure as to the identity of these extra bands.
VAP1 has a predicted molecular weight of 85 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). The observed 100kDa band is consistent with other commercially avaliable antibodies.
ab42885 has not yet been referenced specifically in any publications.