Anti-VAPA antibody (ab176995)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-VAPA antibody
See all VAPA primary antibodies -
Description
Rabbit polyclonal to VAPA -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human VAPA aa 18-204. BC002992
Sequence:DPP TDLKFKGPFT DVVTTNLKLR NPSDRKVCFK VKTTAPRRYC VRPNSGIIDP GSTVTVSVML QPFDYDPNEK SKHKFMVQTI FAPPNTSDME AVWKEAKPDE LMDSKLRCVF EMPNENDKLN DMEPSKAVPL NASKQDGPMP KPHSVSLNDT ETRKLMEECK RLQGEMMKLS EENRHLRDEG LRLR
Database link: Q9P0L0 -
Positive control
- NIH-3T3, 293, HepG2, Fetal brain, fetal stomach lysates. Coloon cancer tissue.
Properties
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Form
Lyophilised:200 ul sterile water -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab176995 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | 1/100 - 1/500. | |
WB | 1/200 - 1/1000. Predicted molecular weight: 28 kDa. |
Target
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Function
May play a role in vesicle trafficking. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.
Contains 1 MSP domain. -
Cellular localization
Endoplasmic reticulum membrane. Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells. - Information by UniProt
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Database links
- Entrez Gene: 516024 Cow
- Entrez Gene: 9218 Human
- Entrez Gene: 30960 Mouse
- Entrez Gene: 58857 Rat
- Omim: 605703 Human
- SwissProt: Q0VCY1 Cow
- SwissProt: Q9P0L0 Human
- SwissProt: Q9WV55 Mouse
see all -
Alternative names
- 33 kDa Vamp-associated protein antibody
- hVAP 33 antibody
- MGC3745 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody (ab176995)
Immunohistochemical staining of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling VAPA with ab176995 at 1/100 dilution showing cytoplasmic staining
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All lanes : Anti-VAPA antibody (ab176995) at 1/500 dilution
Lane 1 : NIH-3T3 cell lysate
Lane 2 : 293 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : Human fetal brain lysate
Lane 5 : Human fetal stomach lysate
Predicted band size: 28 kDa
References
ab176995 has not yet been referenced specifically in any publications.