Overview

  • Product name
  • Description
    Rabbit polyclonal to VAPA
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human VAPA aa 18-204. BC002992
    Sequence:

    DPP TDLKFKGPFT DVVTTNLKLR NPSDRKVCFK VKTTAPRRYC VRPNSGIIDP GSTVTVSVML QPFDYDPNEK SKHKFMVQTI FAPPNTSDME AVWKEAKPDE LMDSKLRCVF EMPNENDKLN DMEPSKAVPL NASKQDGPMP KPHSVSLNDT ETRKLMEECK RLQGEMMKLS EENRHLRDEG LRLR


    Database link: Q9P0L0

  • Positive control
    • NIH-3T3, 293, HepG2, Fetal brain, fetal stomach lysates. Coloon cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab176995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/200 - 1/1000. Predicted molecular weight: 28 kDa.

Target

  • Function
    May play a role in vesicle trafficking.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.
    Contains 1 MSP domain.
  • Cellular localization
    Endoplasmic reticulum membrane. Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • 33 kDa Vamp-associated protein antibody
    • hVAP 33 antibody
    • MGC3745 antibody
    • OTTHUMP00000162362 antibody
    • OTTHUMP00000162363 antibody
    • VAMP (vesicle-associated membrane protein) associated protein A, 33kDa antibody
    • VAMP A antibody
    • VAMP associated protein A antibody
    • VAMP-A antibody
    • VAMP-associated protein A antibody
    • VAP 33 antibody
    • VAP A antibody
    • VAP-33 antibody
    • VAP-A antibody
    • VAP33 antibody
    • Vapa antibody
    • VAPA_HUMAN antibody
    • Vesicle associated membrane protein associated protein A antibody
    • Vesicle-associated membrane protein-associated protein A antibody
    see all

Images

  • Immunohistochemical staining of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling VAPA with ab176995 at 1/100 dilution showing cytoplasmic staining

  • All lanes : Anti-VAPA antibody (ab176995) at 1/500 dilution

    Lane 1 : NIH-3T3 cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Human fetal brain lysate
    Lane 5 : Human fetal stomach lysate

    Predicted band size: 28 kDa

References

ab176995 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HL60)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
HL60
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 20 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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