Anti-VAPA antibody (ab176995)

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Overview

  • Product name

    Anti-VAPA antibody
    See all VAPA primary antibodies

  • Description

    Rabbit polyclonal to VAPA

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow

  • Immunogen

    Recombinant fragment corresponding to Human VAPA aa 18-204. BC002992
    Sequence:

    DPP TDLKFKGPFT DVVTTNLKLR NPSDRKVCFK VKTTAPRRYC VRPNSGIIDP GSTVTVSVML QPFDYDPNEK SKHKFMVQTI FAPPNTSDME AVWKEAKPDE LMDSKLRCVF EMPNENDKLN DMEPSKAVPL NASKQDGPMP KPHSVSLNDT ETRKLMEECK RLQGEMMKLS EENRHLRDEG LRLR


    Database link: Q9P0L0

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • NIH-3T3, 293, HepG2, Fetal brain, fetal stomach lysates. Coloon cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab176995 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500.
WB 1/200 - 1/1000. Predicted molecular weight: 28 kDa.

Target

  • Function

    May play a role in vesicle trafficking.

  • Tissue specificity

    Ubiquitous.

  • Sequence similarities

    Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.
    Contains 1 MSP domain.

  • Cellular localization

    Endoplasmic reticulum membrane. Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 33 kDa Vamp-associated protein antibody
    • hVAP 33 antibody
    • MGC3745 antibody
    • OTTHUMP00000162362 antibody
    • OTTHUMP00000162363 antibody
    • VAMP (vesicle-associated membrane protein) associated protein A, 33kDa antibody
    • VAMP A antibody
    • VAMP associated protein A antibody
    • VAMP-A antibody
    • VAMP-associated protein A antibody
    • VAP 33 antibody
    • VAP A antibody
    • VAP-33 antibody
    • VAP-A antibody
    • VAP33 antibody
    • Vapa antibody
    • VAPA_HUMAN antibody
    • Vesicle associated membrane protein associated protein A antibody
    • Vesicle-associated membrane protein-associated protein A antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody (ab176995)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody (ab176995)

    Immunohistochemical staining of formalin-fixed, paraffin-embedded Human colon carcinoma tissue labeling VAPA with ab176995 at 1/100 dilution showing cytoplasmic staining

  • Western blot - Anti-VAPA antibody (ab176995)
    Western blot - Anti-VAPA antibody (ab176995)
    All lanes : Anti-VAPA antibody (ab176995) at 1/500 dilution

    Lane 1 : NIH-3T3 cell lysate
    Lane 2 : 293 cell lysate
    Lane 3 : HepG2 cell lysate
    Lane 4 : Human fetal brain lysate
    Lane 5 : Human fetal stomach lysate

    Predicted band size: 28 kDa

References

ab176995 has not yet been referenced specifically in any publications.

Publishing research using ab176995? Please let us know so that we can cite the reference in this datasheet.

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Western blot abreview for Anti-VAPA antibody

 Excellent
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HL60)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
HL60
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

Submitted Mar 20 2018

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