Overview

  • Product name

  • Description

    Rabbit polyclonal to VAPA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 29 - 92 of Human VAPA.

  • Positive control

    • 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji or NIH3T3 cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.

Properties

Applications

Our Abpromise guarantee covers the use of ab96584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/3000. Predicted molecular weight: 27 kDa.

Target

  • Function

    May play a role in vesicle trafficking.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.
    Contains 1 MSP domain.
  • Cellular localization

    Endoplasmic reticulum membrane. Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells.
  • Information by UniProt
  • Database links

  • Alternative names

    • 33 kDa Vamp-associated protein antibody
    • hVAP 33 antibody
    • MGC3745 antibody
    • OTTHUMP00000162362 antibody
    • OTTHUMP00000162363 antibody
    • VAMP (vesicle-associated membrane protein) associated protein A, 33kDa antibody
    • VAMP A antibody
    • VAMP associated protein A antibody
    • VAMP-A antibody
    • VAMP-associated protein A antibody
    • VAP 33 antibody
    • VAP A antibody
    • VAP-33 antibody
    • VAP-A antibody
    • VAP33 antibody
    • Vapa antibody
    • VAPA_HUMAN antibody
    • Vesicle associated membrane protein associated protein A antibody
    • Vesicle-associated membrane protein-associated protein A antibody
    see all

Images

  • IHC image of VAPA staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96584, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-VAPA antibody (ab96584) at 1/1000 dilution (12% SDS PAGE)

    Lane 1 : H1299 whole cell lysate
    Lane 2 : NIH3T3 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 27 kDa

References

This product has been referenced in:

  • Deng Q  et al. Vesicle-Associated Membrane Protein-Associated Protein A Is Involved in Androgen Receptor Trafficking in Mouse Sertoli Cells. Int J Endocrinol 2018:4537214 (2018). Read more (PubMed: 29686703) »
See 1 Publication for this product

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