Product nameAnti-VAPA antibody
See all VAPA primary antibodies
DescriptionRabbit polyclonal to VAPA
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Pig, Zebrafish
Synthetic peptide, corresponding to a region within amino acids 29 - 92 of Human VAPA.
- 293T, A431, H1299, HeLa, HepG2, MOLT4, Raji or NIH3T3 cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal cerebral cortex.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab96584 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/500 - 1/3000. Predicted molecular weight: 27 kDa.|
FunctionMay play a role in vesicle trafficking.
Sequence similaritiesBelongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.
Contains 1 MSP domain.
Cellular localizationEndoplasmic reticulum membrane. Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells.
- Information by UniProt
- 33 kDa Vamp-associated protein antibody
- hVAP 33 antibody
- MGC3745 antibody
IHC image of VAPA staining in Human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96584, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-VAPA antibody (ab96584) at 1/1000 dilution (12% SDS PAGE)
Lane 1 : H1299 whole cell lysate
Lane 2 : NIH3T3 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 27 kDa