Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-VAPA antibody [EPR13589(B)] - N-terminal
See all VAPA primary antibodies -
DescriptionRabbit monoclonal [EPR13589(B)] to VAPA - N-terminal
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
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Immunogen
Synthetic peptide within Human VAPA aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9P0L0 -
Positive control
- HeLa, K562, HEK293, HepG2 cell lysates. Human fetal colon and Human ovarian carcinoma tissues.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberEPR13589(B)
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IsotypeIgG
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Research areas
Associated products
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab181067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). | |
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionMay play a role in vesicle trafficking.
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Tissue specificityUbiquitous.
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Sequence similaritiesBelongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.
Contains 1 MSP domain. -
Cellular localizationEndoplasmic reticulum membrane. Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells.
- Information by UniProt
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Database links
- Entrez Gene: 516024 Cow
- Entrez Gene: 9218 Human
- Entrez Gene: 30960 Mouse
- Entrez Gene: 100173641 Orangutan
- Entrez Gene: 58857 Rat
- Omim: 605703 Human
- SwissProt: Q0VCY1 Cow
- SwissProt: Q9P0L0 Human
see all -
Alternative names
- 33 kDa Vamp-associated protein antibody
- hVAP 33 antibody
- MGC3745 antibody
see all
Images
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![Western blot - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)](https://www.abcam.com/ps/products/181/ab181067/Images/ab181067-213295-ab181067WB.jpg)
Western blot - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)All lanes : Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)](https://www.abcam.com/ps/products/181/ab181067/Images/ab181067-213297-ab181067IHC1.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)Immunohistochemical analysis of Human fetal colon tissue, staining VAPA with ab181067 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)](https://www.abcam.com/ps/products/181/ab181067/Images/ab181067-213298-ab181067IHC2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)Immunohistochemical analysis of Human ovarian carcinoma, staining VAPA with ab181067 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Datasheets and documents
References
ab181067 has not yet been referenced specifically in any publications.
