Recombinant Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)
![Western blot - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)](https://www.abcam.com/ps/products/181/ab181067/Images/ab181067-213295-anti-vapa-antibody-epr13589b-n-terminal-western-blot.jpg "Western blot - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR13589(B)] to VAPA - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-VAPA antibody [EPR13589(B)] - N-terminal
See all VAPA primary antibodies -
Description
Rabbit monoclonal [EPR13589(B)] to VAPA - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- HeLa, K562, HEK293, HepG2 cell lysates. Human fetal colon and Human ovarian carcinoma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR13589(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab181067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
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| IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
|---|
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WB
1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa). |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May play a role in vesicle trafficking. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.
Contains 1 MSP domain. -
Cellular localization
Endoplasmic reticulum membrane. Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells. - Information by UniProt
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Database links
- Entrez Gene: 516024 Cow
- Entrez Gene: 9218 Human
- Entrez Gene: 30960 Mouse
- Entrez Gene: 100173641 Orangutan
- Entrez Gene: 58857 Rat
- Omim: 605703 Human
- SwissProt: Q0VCY1 Cow
- SwissProt: Q9P0L0 Human
see all -
Alternative names
- 33 kDa Vamp-associated protein antibody
- hVAP 33 antibody
- MGC3745 antibody
see all
Images
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Western blot - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)All lanes : Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)](https://www.abcam.com/ps/products/181/ab181067/Images/ab181067-213297-anti-vapa-antibody-epr13589b-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)Immunohistochemical analysis of Human fetal colon tissue, staining VAPA with ab181067 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)](https://www.abcam.com/ps/products/181/ab181067/Images/ab181067-213298-anti-vapa-antibody-epr13589b-n-terminal-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)Immunohistochemical analysis of Human ovarian carcinoma, staining VAPA with ab181067 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)](https://www.abcam.com/ps/products/181/ab181067/Images/ab181067-4-benefits-of-recombinant-antibodies.png)
Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab181067 has been referenced in 1 publication.
- Papadopoulou AA et al. Signal Peptide Peptidase-Like 2c (SPPL2c) impairs vesicular transport and cleavage of SNARE proteins. EMBO Rep 20:N/A (2019). PubMed: 30733281