Product nameAnti-VAPA antibody [EPR13589(B)] - N-terminal
See all VAPA primary antibodies
DescriptionRabbit monoclonal [EPR13589(B)] to VAPA - N-terminal
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Orangutan
Synthetic peptide within Human VAPA aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q9P0L0
- HeLa, K562, HEK293, HepG2 cell lysates. Human fetal colon and Human ovarian carcinoma tissues.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab181067 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionMay play a role in vesicle trafficking.
Sequence similaritiesBelongs to the VAMP-associated protein (VAP) (TC 9.B.17) family.
Contains 1 MSP domain.
Cellular localizationEndoplasmic reticulum membrane. Present in the plasma membrane and in intracellular vesicles, together with SNARE proteins. May also associate with the cytoskeleton. Colocalizes with OCLN at the tight junction in polarized epithelial cells.
- Information by UniProt
- 33 kDa Vamp-associated protein antibody
- hVAP 33 antibody
- MGC3745 antibody
All lanes : Anti-VAPA antibody [EPR13589(B)] - N-terminal (ab181067) at 1/20000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HEK293 cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Immunohistochemical analysis of Human fetal colon tissue, staining VAPA with ab181067 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
Immunohistochemical analysis of Human ovarian carcinoma, staining VAPA with ab181067 at 1/100 dilution. Detected using HRP Polymer for Rabbit IgG and counter-stained using hematoxylin.
ab181067 has not yet been referenced specifically in any publications.