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    vapb-antibody-ab103638.pdf

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Signal Transduction Protein Trafficking Vesicle Transport Regulation
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Anti-VAPB antibody (ab103638)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (1)

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Western blot - Anti-VAPB antibody (ab103638)
  • Western blot - Anti-VAPB antibody (ab103638)
  • Western blot - Anti-VAPB antibody (ab103638)

Key features and details

  • Rabbit polyclonal to VAPB
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human VAPB protein (ab101214)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-VAPB antibody
    See all VAPB primary antibodies
  • Description

    Rabbit polyclonal to VAPB
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Full length Human VAPB protein (NP_004729.1).

  • Positive control

    • Mouse lung tissue lysate; HepG2 cell lysate; VAPB transfected 293T cell lysate
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.4
    Constituent: 2.68% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human VAPB protein (ab101214)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab103638 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (2)
1/500 - 1/1000. Predicted molecular weight: 27 kDa.
Notes
WB
1/500 - 1/1000. Predicted molecular weight: 27 kDa.

Target

  • Relevance

    VAPB contains 1 MSP domain and it may play a role in vesicle trafficking. Defects in VAPB are a cause of proximal adult autosomal dominant spinal muscular atrophy [MIM:182980]; also called late onset spinal muscular atrophy Finkel type. Spinal muscular atrophies are neurodegenerative disorders characterized by degeneration of lower motor neurons, leading to progressive paralysis muscular atrophy. This form is a late adult onset form of the disease (after age 20 years). The patients show a benign course, most of them remaining ambulatory 10 to 40 years after clinical onset.
  • Cellular localization

    Cell membrane; Single-pass type IV membrane. Intracytoplasmic membrane; Single-pass type IV membrane protein. Note: Present in the plasma membrane and in intracellular vesicles.
  • Database links

    • Entrez Gene: 9217 Human
    • Entrez Gene: 56491 Mouse
    • Omim: 605704 Human
    • SwissProt: O95292 Human
    • SwissProt: Q9QY76 Mouse
    • Unigene: 182625 Human
    • Unigene: 260456 Mouse
    • Alternative names

      • ALS 8 antibody
      • ALS8 antibody
      • D2Abb2e antibody
      • UNQ484/PRO983 antibody
      • Vamp 33b antibody
      • VAMP associated 33 kDa protein antibody
      • VAMP associated protein B and C antibody
      • VAMP associated protein B antibody
      • VAMP associated protein B/C antibody
      • VAMP associated protein C antibody
      • VAMP B antibody
      • VAMP B VAMP C antibody
      • VAMP B/VAMP C antibody
      • VAMP C antibody
      • VAMP vesicle associated membrane protein associated protein B and C antibody
      • Vamp33b antibody
      • VAMPB antibody
      • VAMPB/VAMPC antibody
      • VAMPC antibody
      • VAP 33b antibody
      • VAP B antibody
      • VAP B/VAP C antibody
      • VAP C antibody
      • VAP33b antibody
      • VAPB/VAPC antibody
      • VAPC antibody
      • Vesicle associated membrane protein associated protein B and C antibody
      • Vesicle associated membrane protein associated protein B/C antibody
      see all

    Images

    • Western blot - Anti-VAPB antibody (ab103638)
      Western blot - Anti-VAPB antibody (ab103638)
      Anti-VAPB antibody (ab103638) at 1/500 dilution + Mouse lung tissue lysate at 50 µg

      Predicted band size: 27 kDa

    • Western blot - Anti-VAPB antibody (ab103638)
      Western blot - Anti-VAPB antibody (ab103638)
      Anti-VAPB antibody (ab103638) at 1/500 dilution + HepG2 cell lysate at 50 µg

      Predicted band size: 27 kDa

    • Western blot - Anti-VAPB antibody (ab103638)
      Western blot - Anti-VAPB antibody (ab103638)
      All lanes : Anti-VAPB antibody (ab103638) at 1/500 dilution

      Lane 1 : VAPB transfected 293T cell line lysate
      Lane 2 : Non-transfected 293T cell line lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 27 kDa

    Protocols

    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab103638? Please let us know so that we can cite the reference in this datasheet.

    ab103638 has been referenced in 1 publication.

    • Papadopoulou AA  et al. Signal Peptide Peptidase-Like 2c (SPPL2c) impairs vesicular transport and cleavage of SNARE proteins. EMBO Rep 20:N/A (2019). PubMed: 30733281

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-VAPB antibody

    Inconclusive
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Dopaminergic Neurons & HCT116)
    Gel Running Conditions
    Reduced Denaturing (NuPage 4-12% MES buffer)
    Loading amount
    30 µg
    Specification
    Dopaminergic Neurons & HCT116
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 16 2020

    Western blot abreview for Anti-VAPB antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (colon cancer)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    colon cancer
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 16 2020

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