Anti-VAPB antibody (ab103638)
Key features and details
- Rabbit polyclonal to VAPB
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-VAPB antibody
See all VAPB primary antibodies -
Description
Rabbit polyclonal to VAPB -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Full length Human VAPB protein (NP_004729.1).
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Positive control
- Mouse lung tissue lysate; HepG2 cell lysate; VAPB transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Constituent: 2.68% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab103638 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/500 - 1/1000. Predicted molecular weight: 27 kDa.
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| Notes |
|---|
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WB
1/500 - 1/1000. Predicted molecular weight: 27 kDa. |
Target
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Relevance
VAPB contains 1 MSP domain and it may play a role in vesicle trafficking. Defects in VAPB are a cause of proximal adult autosomal dominant spinal muscular atrophy [MIM:182980]; also called late onset spinal muscular atrophy Finkel type. Spinal muscular atrophies are neurodegenerative disorders characterized by degeneration of lower motor neurons, leading to progressive paralysis muscular atrophy. This form is a late adult onset form of the disease (after age 20 years). The patients show a benign course, most of them remaining ambulatory 10 to 40 years after clinical onset. -
Cellular localization
Cell membrane; Single-pass type IV membrane. Intracytoplasmic membrane; Single-pass type IV membrane protein. Note: Present in the plasma membrane and in intracellular vesicles. -
Database links
- Entrez Gene: 9217 Human
- Entrez Gene: 56491 Mouse
- Omim: 605704 Human
- SwissProt: O95292 Human
- SwissProt: Q9QY76 Mouse
- Unigene: 182625 Human
- Unigene: 260456 Mouse
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Alternative names
- ALS 8 antibody
- ALS8 antibody
- D2Abb2e antibody
see all
Images
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Western blot - Anti-VAPB antibody (ab103638)Anti-VAPB antibody (ab103638) at 1/500 dilution + Mouse lung tissue lysate at 50 µg
Predicted band size: 27 kDa -
Western blot - Anti-VAPB antibody (ab103638)Anti-VAPB antibody (ab103638) at 1/500 dilution + HepG2 cell lysate at 50 µg
Predicted band size: 27 kDa -
Western blot - Anti-VAPB antibody (ab103638)All lanes : Anti-VAPB antibody (ab103638) at 1/500 dilution
Lane 1 : VAPB transfected 293T cell line lysate
Lane 2 : Non-transfected 293T cell line lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 27 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab103638 has been referenced in 1 publication.
- Papadopoulou AA et al. Signal Peptide Peptidase-Like 2c (SPPL2c) impairs vesicular transport and cleavage of SNARE proteins. EMBO Rep 20:N/A (2019). PubMed: 30733281