Overview

  • Product name

  • Description

    Rabbit polyclonal to VAPB
  • Host species

    Rabbit
  • Specificity

    ab196487 detects endogenous level of total VAPB protein.
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Recombinant fragment within Human VAPB (internal sequence). The exact sequence is proprietary.
    Database link: O95292

  • Positive control

    • BT474, SW480, HepG2, H460 and Raji cell extracts; mouse brain and intestine tissue extracts; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab196487 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/200.
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 27 kDa.

Target

  • Relevance

    VAPB contains 1 MSP domain and it may play a role in vesicle trafficking. Defects in VAPB are a cause of proximal adult autosomal dominant spinal muscular atrophy [MIM:182980]; also called late onset spinal muscular atrophy Finkel type. Spinal muscular atrophies are neurodegenerative disorders characterized by degeneration of lower motor neurons, leading to progressive paralysis muscular atrophy. This form is a late adult onset form of the disease (after age 20 years). The patients show a benign course, most of them remaining ambulatory 10 to 40 years after clinical onset.
  • Cellular localization

    Cell membrane; Single-pass type IV membrane. Intracytoplasmic membrane; Single-pass type IV membrane protein. Note: Present in the plasma membrane and in intracellular vesicles.
  • Database links

  • Alternative names

    • ALS 8 antibody
    • ALS8 antibody
    • D2Abb2e antibody
    • UNQ484/PRO983 antibody
    • Vamp 33b antibody
    • VAMP associated 33 kDa protein antibody
    • VAMP associated protein B and C antibody
    • VAMP associated protein B antibody
    • VAMP associated protein B/C antibody
    • VAMP associated protein C antibody
    • VAMP B antibody
    • VAMP B VAMP C antibody
    • VAMP B/VAMP C antibody
    • VAMP C antibody
    • VAMP vesicle associated membrane protein associated protein B and C antibody
    • Vamp33b antibody
    • VAMPB antibody
    • VAMPB/VAMPC antibody
    • VAMPC antibody
    • VAP 33b antibody
    • VAP B antibody
    • VAP B/VAP C antibody
    • VAP C antibody
    • VAP33b antibody
    • VAPB/VAPC antibody
    • VAPC antibody
    • Vesicle associated membrane protein associated protein B and C antibody
    • Vesicle associated membrane protein associated protein B/C antibody
    see all

Images

  • All lanes : Anti-VAPB antibody (ab196487) at 1/500 dilution

    Lane 1 : BT474 cell extract
    Lane 2 : SW480 cell extract
    Lane 3 : HepG2 cell extract
    Lane 4 : H460 cell extract
    Lane 5 : Raji cell extract
    Lane 6 : Mouse brain tissue extract
    Lane 7 : Mouse intestine tissue extract

    Predicted band size: 27 kDa

  • Immunofluorescence analysis of HeLa cells labeling VAPB (green) with ab196487 at 1/50 dilution. Nuclei were counterstained with DAPI (blue).

References

ab196487 has not yet been referenced specifically in any publications.

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