Key features and details
- Rabbit polyclonal to VAPB
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-VAPB antibody
See all VAPB primary antibodies
DescriptionRabbit polyclonal to VAPB
Specificityab196487 detects endogenous level of total VAPB protein.
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat
Recombinant fragment within Human VAPB (internal sequence). The exact sequence is proprietary.
Database link: O95292
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
PBS is without Mg2+ and Ca2+.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab196487 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 27 kDa.|
RelevanceVAPB contains 1 MSP domain and it may play a role in vesicle trafficking. Defects in VAPB are a cause of proximal adult autosomal dominant spinal muscular atrophy [MIM:182980]; also called late onset spinal muscular atrophy Finkel type. Spinal muscular atrophies are neurodegenerative disorders characterized by degeneration of lower motor neurons, leading to progressive paralysis muscular atrophy. This form is a late adult onset form of the disease (after age 20 years). The patients show a benign course, most of them remaining ambulatory 10 to 40 years after clinical onset.
Cellular localizationCell membrane; Single-pass type IV membrane. Intracytoplasmic membrane; Single-pass type IV membrane protein. Note: Present in the plasma membrane and in intracellular vesicles.
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All lanes : Anti-VAPB antibody (ab196487) at 1/500 dilution
Lane 1 : BT474 cell extract
Lane 2 : SW480 cell extract
Lane 3 : HepG2 cell extract
Lane 4 : H460 cell extract
Lane 5 : Raji cell extract
Lane 6 : Mouse brain tissue extract
Lane 7 : Mouse intestine tissue extract
Predicted band size: 27 kDa
Immunofluorescence analysis of HeLa cells labeling VAPB (green) with ab196487 at 1/50 dilution. Nuclei were counterstained with DAPI (blue).
ab196487 has not yet been referenced specifically in any publications.