Anti-Varicella Zoster Virus IE62 antibody [62B] - BSA and Azide free (ab212015)

Overview

  • Product name

    Anti-Varicella Zoster Virus IE62 antibody [62B] - BSA and Azide free
    See all Varicella Zoster Virus IE62 primary antibodies
  • Description

    Mouse monoclonal [62B] to Varicella Zoster Virus IE62 - BSA and Azide free
  • Host species

    Mouse
  • Specificity

    This antibody reacts with IE62 of VZV.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Tissue, cells or virus corresponding to Human Herpesvirus Varicella Zoster Virus IE62. Varicella-zoster virus (Human herpesvirus 3) Oka strain (vaccine strain).
    Database link: Q8AZM1

  • Positive control

    • Varicella Zoster Virus strain pOka infected MRC5 cell lysate; Varicella Zoster Virus-infected MRC5 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 50% Glycerol, 50% PBS

    Azide- and carrier-free.
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    ab212015 was produced by hybridoma grown in serum-free medium and purified by a proprietary chromatography procedure under mild conditions. 90~95% pure by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    62B
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab212015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/5000. Predicted molecular weight: 140 kDa.
ICC/IF 1/50 - 1/100.

Target

  • Relevance

    Varicella Zoster Virus (VZV), a member of the human herpes virus family, causes two distinct clinical manifestations: childhood chickenpox(Varicella) and shingles (zoster). Varicella is the outcome of the primary infection with VZV, whereas, zoster is the result of VZV reactivation from latently infected sensory ganglia which occurs predominantly in aging and immunosuppressed individuals. VZV is closely related to the herpes simplex viruses (HSV), sharing much genome homology. The known envelope glycoproteins (gB, gC, gE, gH, gI, gK, gL) correspond with those in HSV, however there is no equivalent of HSV gD. VZV virons are spherical and 150-200 nm in diameter. Its lipid envelope encloses the nucleocapsid of 162 capsomeres arranged in a hexagonal form. Its DNA is a single linear, double strand molecule, 125,000 nt long. The immediate-early protein IE62 is a major component of virus particles and initiates transcription from its own and other VZV gene promoters, acting synergistically with several viral and cellular transactivating proteins.
  • Alternative names

    • HHV3 antibody
    • Human Herpesvirus 3 antibody
    • IE62 antibody
    • ORF 62 antibody
    • Varicella Zoster virus antibody
    • VZV antibody
    see all

Images

  • Immuofluorescent analysis of Varicella Zoster Virus (VZV) -infected MRC5 cells labeling Varicella Zoster Virus IE62 with ab212015 at 1/100 dilution, followed by Alexa Fluor 488 donkey anti-mouse IgG [H+L] at 1/200 dilution (green). Nuclei were stained with Hoechst 33342 (blue).

  • All lanes : Anti-Varicella Zoster Virus IE62 antibody [62B] - BSA and Azide free (ab212015) at 1/5000 dilution

    Lane 1 : VZV strain pOka infected MRC5 cell lysate
    Lane 2 : MRC5 cell lysate (uninfected negative control)

    Predicted band size: 140 kDa

References

ab212015 has not yet been referenced specifically in any publications.

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