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  1. Link

    varicella-zoster-virus-ie62-antibody-62b-bsa-and-azide-free-ab212015.pdf

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Microbiology Organism Virus DNA Virus double stranded DNA Virus HHV
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Anti-Varicella Zoster Virus IE62 antibody [62B] - BSA and Azide free (ab212015)

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Immunocytochemistry/ Immunofluorescence - Anti-Varicella Zoster Virus IE62 antibody [62B] - BSA and Azide free (ab212015)
  • Western blot - Anti-Varicella Zoster Virus IE62 antibody [62B] - BSA and Azide free (ab212015)

Key features and details

  • Mouse monoclonal [62B] to Varicella Zoster Virus IE62
  • Suitable for: WB, ICC/IF
  • Reacts with: Varicella zoster virus
  • Isotype: IgG1

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Overview

  • Product name

    Anti-Varicella Zoster Virus IE62 antibody [62B] - BSA and Azide free
    See all Varicella Zoster Virus IE62 primary antibodies
  • Description

    Mouse monoclonal [62B] to Varicella Zoster Virus IE62
  • Host species

    Mouse
  • Specificity

    This antibody reacts with IE62 of VZV.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Varicella zoster virus
  • Immunogen

    Tissue, cells or virus corresponding to Human Herpesvirus Varicella Zoster Virus IE62. Varicella-zoster virus (Human herpesvirus 3) Oka strain (vaccine strain).
    Database link: Q8AZM1

  • Positive control

    • Varicella Zoster Virus strain pOka infected MRC5 cell lysate; Varicella Zoster Virus-infected MRC5 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6
    Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS

    Azide- and carrier-free.
  • Concentration information loading...
  • Purity

    Proprietary Purification
  • Purification notes

    ab212015 was produced by hybridoma grown in serum-free medium and purified by a proprietary chromatography procedure under mild conditions. 90~95% pure by SDS-PAGE.
  • Clonality

    Monoclonal
  • Clone number

    62B
  • Isotype

    IgG1
  • Light chain type

    kappa
  • Research areas

    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • HHV
    • Microbiology
    • Organism
    • Virus
    • DNA Virus
    • double stranded DNA Virus
    • Other dsDNA viruses

Associated products

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab212015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/2000 - 1/5000. Predicted molecular weight: 140 kDa.
ICC/IF
1/50 - 1/100.
Notes
WB
1/2000 - 1/5000. Predicted molecular weight: 140 kDa.
ICC/IF
1/50 - 1/100.

Target

  • Relevance

    Varicella Zoster Virus (VZV), a member of the human herpes virus family, causes two distinct clinical manifestations: childhood chickenpox(Varicella) and shingles (zoster). Varicella is the outcome of the primary infection with VZV, whereas, zoster is the result of VZV reactivation from latently infected sensory ganglia which occurs predominantly in aging and immunosuppressed individuals. VZV is closely related to the herpes simplex viruses (HSV), sharing much genome homology. The known envelope glycoproteins (gB, gC, gE, gH, gI, gK, gL) correspond with those in HSV, however there is no equivalent of HSV gD. VZV virons are spherical and 150-200 nm in diameter. Its lipid envelope encloses the nucleocapsid of 162 capsomeres arranged in a hexagonal form. Its DNA is a single linear, double strand molecule, 125,000 nt long. The immediate-early protein IE62 is a major component of virus particles and initiates transcription from its own and other VZV gene promoters, acting synergistically with several viral and cellular transactivating proteins.
  • Alternative names

    • HHV3 antibody
    • Human Herpesvirus 3 antibody
    • IE62 antibody
    • ORF 62 antibody
    • Varicella Zoster virus antibody
    • VZV antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Varicella Zoster Virus IE62 antibody [62B] - BSA and Azide free (ab212015)
    Immunocytochemistry/ Immunofluorescence - Anti-Varicella Zoster Virus IE62 antibody [62B] - BSA and Azide free (ab212015)

    Immuofluorescent analysis of Varicella Zoster Virus (VZV) -infected MRC5 cells labeling Varicella Zoster Virus IE62 with ab212015 at 1/100 dilution, followed by Alexa Fluor 488 donkey anti-mouse IgG [H+L] at 1/200 dilution (green). Nuclei were stained with Hoechst 33342 (blue).

  • Western blot - Anti-Varicella Zoster Virus IE62 antibody [62B] - BSA and Azide free (ab212015)
    Western blot - Anti-Varicella Zoster Virus IE62 antibody [62B] - BSA and Azide free (ab212015)
    All lanes : Anti-Varicella Zoster Virus IE62 antibody [62B] - BSA and Azide free (ab212015) at 1/5000 dilution

    Lane 1 : VZV strain pOka infected MRC5 cell lysate
    Lane 2 : MRC5 cell lysate (uninfected negative control)

    Predicted band size: 140 kDa

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (0)

Publishing research using ab212015? Please let us know so that we can cite the reference in this datasheet.

ab212015 has not yet been referenced specifically in any publications.

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