Key features and details
- Rabbit polyclonal to VARP
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-VARP antibody
DescriptionRabbit polyclonal to VARP
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Gorilla, Orangutan
Synthetic peptide, corresponding to a region between amino acids 1000-1050 of Human VARP (NP_115515.2).
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab108216 was affinity purified using an epitope specific to VARP immobilized on solid support.
Our Abpromise guarantee covers the use of ab108216 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 117 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionMay be a Rab21 guanine exchange factor and regulate endosome dynamics.
Sequence similaritiesContains 11 ANK repeats.
Contains 1 VPS9 domain.
Cellular localizationCytoplasm. Mainly localized to early endosomes in the perinuclear region.
- Information by UniProt
- AA408090 antibody
- Ankrd27 antibody
- Ankyrin repeat domain 27 (VPS9 domain) antibody
All lanes : Anti-VARP antibody (ab108216) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 117 kDa
Exposure time: 3 minutes
Detection of VARP by Western Blot of Immunprecipitate.
Lane 1: ab108216 at 1µg/ml staining VARP in HeLa whole cell lysate immunoprecipitated using ab108216 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: Control IgG
Detection: Chemiluminescence with exposure time of 30 seconds.
ab108216 has been referenced in 1 publication.
- McGough IJ et al. Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma-membrane recycling. J Cell Sci 127:4940-53 (2014). PubMed: 25278552