Overview

  • Product name

    Anti-VARP antibody
  • Description

    Rabbit polyclonal to VARP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 1000-1050 of Human VARP (NP_115515.2).

  • Positive control

    • HeLa and 293T whole cell lysates IF/ICC: HeLa cell line.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: Tris citrate/phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab108216 was affinity purified using an epitope specific to VARP immobilized on solid support.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/2000 - 1/10000. Predicted molecular weight: 117 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    May be a Rab21 guanine exchange factor and regulate endosome dynamics.
  • Sequence similarities

    Contains 11 ANK repeats.
    Contains 1 VPS9 domain.
  • Cellular localization

    Cytoplasm. Mainly localized to early endosomes in the perinuclear region.
  • Information by UniProt
  • Database links

  • Alternative names

    • AA408090 antibody
    • Ankrd27 antibody
    • Ankyrin repeat domain 27 (VPS9 domain) antibody
    • Ankyrin repeat domain-containing protein 27 antibody
    • ANR27_HUMAN antibody
    • BC016493 antibody
    • D330003H11Rik antibody
    • DKFZp434L0718 antibody
    • FLJ00040 antibody
    • MGC25907 antibody
    • PP12899 antibody
    • VPS9 ankyrin repeat protein antibody
    • VPS9 domain-containing protein antibody
    see all

Images

  • All lanes : Anti-VARP antibody (ab108216) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 117 kDa


    Exposure time: 3 minutes
  • ab108216 stained HeLa cells. The cells were 4% formaldehyde fixed for 10 minutes at room temperature and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab108216 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed (ab150081) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Detection of VARP by Western Blot of Immunprecipitate.
    Lane 1: ab108216 at 1µg/ml staining VARP in HeLa whole cell lysate immunoprecipitated using ab108216 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: Control IgG
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

This product has been referenced in:

  • McGough IJ  et al. Identification of molecular heterogeneity in SNX27-retromer-mediated endosome-to-plasma-membrane recycling. J Cell Sci 127:4940-53 (2014). Read more (PubMed: 25278552) »
See 1 Publication for this product

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