Anti-VASA/VAS antibody (ab209710)
Key features and details
- Rabbit polyclonal to VASA/VAS
- Suitable for: IHC - Wholemount, WB
- Reacts with: Zebrafish
- Isotype: IgG
Overview
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Product name
Anti-VASA/VAS antibody -
Description
Rabbit polyclonal to VASA/VAS -
Host species
Rabbit -
Tested applications
Suitable for: IHC - Wholemount, WBmore details -
Species reactivity
Reacts with: Zebrafish -
Immunogen
Recombinant fragment within Zebrafish VASA/VAS aa 199-526. The exact sequence is proprietary.
Database link: O42107 -
Positive control
- Zebrafish embryos; 7 days-post-fertilization medaka embryo; Zebrafish 10 hpf lysate/extract.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab209710 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC - Wholemount |
1/100 - 1/500.
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WB |
1/500 - 1/3000. Predicted molecular weight: 77 kDa.
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Notes |
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IHC - Wholemount
1/100 - 1/500. |
WB
1/500 - 1/3000. Predicted molecular weight: 77 kDa. |
Target
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Relevance
VASA is required only in the female germline. It is important for oocyte formation and in the specification of the posterior structures of the embryo. -
Database links
- Entrez Gene: 30263 Zebrafish
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Alternative names
- ATP dependent RNA helicase vasa antibody
- DmRH25 antibody
- VAS antibody
- VASA antibody
Images
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Anti-VASA/VAS antibody (ab209710) at 1/2000 dilution + Zebrafish 10 hpf lysate/extract at 30 µg
Predicted band size: 77 kDa7.5 % SDS-PAGE
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Immunohistochemical analysis (whole mount) of paraformaldehyde-fixed Zebrafish embryo labeling VASA/VAS (primordial germ cell) with ab209710 at 1/200 dilution.
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Immunohistochemical analysis (whole mount) of Zebrafish 7 days-post-fertilization medaka embryo labeling VASA/VAS with ab209710 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab209710 has been referenced in 8 publications.
- Arias Padilla LF et al. Cystic proliferation of germline stem cells is necessary to reproductive success and normal mating behavior in medaka. Elife 10:N/A (2021). PubMed: 33646121
- Vong YH et al. The RNA-binding protein Igf2bp3 is critical for embryonic and germline development in zebrafish. PLoS Genet 17:e1009667 (2021). PubMed: 34214072
- Filanti B et al. Early germline differentiation in bivalves: TDRD7 as a candidate investigational unit for Ruditapes philippinarum germ granule assembly. Histochem Cell Biol 156:19-34 (2021). PubMed: 33770286
- Zupa R et al. Meagre Argyrosomus regius (Asso, 1801) Stem Spermatogonia: Histological Characterization, Immunostaining, In Vitro Proliferation, and Cryopreservation. Animals (Basel) 10:N/A (2020). PubMed: 32423131
- Lei JH et al. Cytotoxicity of nonylphenol on spermatogonial stem cells via phosphatidylinositol-3-kinase/protein kinase B/mammalian target of rapamycin pathway. World J Stem Cells 12:500-513 (2020). PubMed: 32742567
- Cavelier P et al. Early gametogenesis in the Pacific oyster: new insights using stem cell and mitotic markers. J Exp Biol 220:3988-3996 (2017). PubMed: 28860120
- Li B et al. Bovine male germline stem-like cells cultured in serum- and feeder-free medium. Cytotechnology 68:2145-57 (2016). PubMed: 26883918
- Ye H et al. Identification and sexually dimorphic expression of vasa isoforms in Dabry's sturgeon (Acipenser dabryanus), and functional analysis of vasa 3'-untranslated region. Cell Tissue Res 366:203-18 (2016). PubMed: 27184950