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  1. Link

    vascular-amyloid-1-42-antibody-moc31-conformation-specific-ab201059.pdf

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Neuroscience Neurology process Neurodegenerative disease Alzheimer's disease Amyloid
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RecombinantRabMAb

Recombinant Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)

  • Datasheet
  • Certificate of Compliance
Reviews (2) Submit a question References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)
  • Dot Blot - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)
  • Immunohistochemistry - Free Floating - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)
  • Dot Blot - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)
  • Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [mOC31] to vascular Amyloid 1-42 - Conformation-Specific
  • Suitable for: Dot blot, IHC-P, IHC-FrFl
  • Reacts with: Mouse, Human

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

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Overview

  • Product name

    Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific
    See all vascular Amyloid 1-42 primary antibodies
  • Description

    Rabbit monoclonal [mOC31] to vascular Amyloid 1-42 - Conformation-Specific
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Dot blot, IHC-P, IHC-FrFlmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Other Immunogen Type corresponding to Human vascular Amyloid 1-42. Amyloid beta 1-42 fibrils were used as the immunogen.
    Database link: P05067

  • Positive control

    • beta Amyloid (Aß) 1-42 IHC-P: FFPE human brain Alzheimer
  • General notes

    This antibody was developed as part of a collaboration between Abcam and Professor Charles Glabe, UC Irvine.

    ab201059 recognizes a conformation-specific discontinuous epitope of beta Amyloid that maps to segments EFGRHSGY and ED (Hatami et al. 2014). It specifically recognizes a subset of vascular amyloid that does not colocalize with thioflavin S (Hatami et al. 2014, McLean et al. 2013).

    For further information on the immunogen, please refer to Hatami et al. 2014 and Kayed et al. 2007.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    mOC31
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Alzheimer's disease
    • Amyloid

Associated products

  • Alternative Versions

    • Anti-vascular Amyloid 1-42 antibody [mOC31] - BSA and Azide free (ab251334)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)

Applications

Our Abpromise guarantee covers the use of ab201059 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot 1/100.
IHC-P 1/250 - 1/500. Do not perform antigen retrieval.
IHC-FrFl Use at an assay dependent concentration.

Target

  • Alternative names

    • A4 antibody
    • A4_HUMAN antibody
    • AAA antibody
    • ABETA antibody
    • ABPP antibody
    • AICD-50 antibody
    • AICD-57 antibody
    • AICD-59 antibody
    • AID(50) antibody
    • AID(57) antibody
    • AID(59) antibody
    • Alzheimer disease amyloid protein antibody
    • Amyloid intracellular domain 50 antibody
    • Amyloid intracellular domain 57 antibody
    • Amyloid intracellular domain 59 antibody
    • APP antibody
    • APPI antibody
    • Beta amyloid protein 42 antibody
    • Beta APP42 antibody
    • Beta-APP40 antibody
    • Beta-APP42 antibody
    • C31 antibody
    • Cerebral vascular amyloid peptide antibody
    • CVAP antibody
    • Gamma-CTF(50) antibody
    • Gamma-CTF(57) antibody
    • Gamma-CTF(59) antibody
    • PN-II antibody
    • PreA4 antibody
    • Protease nexin-II antibody
    • S-APP-alpha antibody
    • S-APP-beta antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)

    IHC image of beta Amyloid staining in a formalin fixed paraffin embedded human Alzheimer brain tissue section, performed on a Leica Bond™ system using the standard protocol F. No antigen retrieval was performed prior to staining. The section was incubated with ab201090 at 1/500 dilution for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

     Image showing staining of vascular Amyloid as described in Hatami et al. 2014

  • Dot Blot - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)
    Dot Blot - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)

    Dot blot analysis of beta Amyloid 1-42 labeled with ab201059 at 1/100 dilution.
    Lane 1: beta Amyloid (Aβ) 1-40.
    Lane 2: beta Amyloid (Aβ) 1-42.
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/5000 dilution was used as secondary antibody.
    Blocking and diluting buffer: 5% NFDM/TBST.
    Exposure time: 1 minute.

    Antibody reactivity was assessed using a dot blot, which is a non-quantitative method that maintains the native conformation of beta Amyloid. Beta Amyloid 1-40 and 1-42 peptides underwent the following aggregation conditions before being spotted onto a nitrocellulose membrane and detected using ab201059:
    Monomers: 0.3 mg of beta Amyloid peptide was dissolved in 30 µl 100 mM NaOH and incubated at room temperature for 10 minutes. It was then diluted with 970 µl of 1% SDS and boiled for five minutes.
    Oligomers: 0.3 mg of beta Amyloid peptide was dissolved in 30 µl 100 mM NaOH and incubated at room temperature for 10 minutes. It was then diluted with 970 µl of 10 mM phosphate buffer pH 7.4 containing 0.02% sodium azide and incubated at room temperature for four days.
    Fibrils: 0.3 mg of beta Amyloid peptide was dissolved in 1 ml 50% hexafluoroisopropanol (HFIP) with 0.02% sodium azide. It was then stirred constantly for nine days; the first seven with a cap on and the final two with the cap removed to allow evaporation of the HFIP. Fibrils were then sedimented at 20,000 rpm in a microcentrifuge for 20 minutes and resuspended in 1 ml of PBS + 0.02% sodium azide.

     

  • Immunohistochemistry - Free Floating - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)
    Immunohistochemistry - Free Floating - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)Image courtesy of Professor Charles Glabe, UC Irvine

    Immunohistochemical staining of human brain tissue from a patient with a diagnosis of Alzheimers disease, male, 81 years, 5 hour post mortem index, tangle stage 5, plaque stage B, mini mental status exam score 12. Sections were cut using a vibratome. No antigen retrieval was performed. Free floating sections were stained using using ab201059 at a dilution of 50 ng/mL. The secondary antibody used was a biotinylated goat anti-rabbit at a dilution of 1/225, which was blocked with normal goat serum. The sample was visualized using ABC solution (1 hour incubation) followed by 1-4 minutes of DAB. The sample was mounted and allowed to dry overnight, followed by dehydration in increasingly concentrated ethanol solutions.

  • Dot Blot - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)
    Dot Blot - Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)

    Negative control (secondary ab only):
    Lane 1: beta Amyloid (Aβ) 1-40.
    Lane 2: beta Amyloid (Aβ) 1-42.

    Primary antibody omitted.
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/5000 dilution was used as secondary antibody.
    Blocking and diluting buffer: 5% NFDM/TBST.
    Exposure time: 30 seconds.

  • Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)
    Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific (ab201059)

Protocols

  • Immunohistochemistry protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (6)

    Publishing research using ab201059? Please let us know so that we can cite the reference in this datasheet.

    ab201059 has been referenced in 6 publications.

    • Paull ML  et al. A general approach for predicting protein epitopes targeted by antibody repertoires using whole proteomes. PLoS One 14:e0217668 (2019). PubMed: 31490930
    • Liu TC  et al. Highly sensitive/selective 3D nanostructured immunoparticle-based interface on a multichannel sensor array for detecting amyloid-beta in Alzheimer's disease. Theranostics 8:4210-4225 (2018). PubMed: 30128048
    • Hatami A  et al. Familial Alzheimer's Disease Mutations within the Amyloid Precursor Protein Alter the Aggregation and Conformation of the Amyloid-ß Peptide. J Biol Chem 292:3172-3185 (2017). PubMed: 28049728
    • Hatami A  et al. Monoclonal antibodies against Aß42 fibrils distinguish multiple aggregation state polymorphisms in vitro and in Alzheimer disease brain. J Biol Chem 289:32131-43 (2014). PubMed: 25281743
    • McLean D  et al. Positron emission tomography imaging of fibrillar parenchymal and vascular amyloid-ß in TgCRND8 mice. ACS Chem Neurosci 4:613-23 (2013). PubMed: 23509918
    • Heine M  et al. High interstitial fluid pressure is associated with low tumour penetration of diagnostic monoclonal antibodies applied for molecular imaging purposes. PLoS One 7:e36258 (2012). PubMed: 22590529

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Rat Tissue sections (Brain)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.5% Triton-X PBS
    Specification
    Brain
    Fixative
    Formaldehyde
    Read More

    Sumit Sarkar

    Verified customer

    Submitted Jun 20 2016

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-vascular Amyloid 1-42 antibody [mOC31] - Conformation-Specific

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.1 M PBS with 1% Triton X
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    Dr. Ruma Raha-Chowdhury

    Verified customer

    Submitted Feb 05 2016

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