Product nameAnti-VASH1 antibody - C-terminal
See all VASH1 primary antibodies
DescriptionRabbit polyclonal to VASH1 - C-terminal
Tested applicationsSuitable for: WB, ICC/IF, IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human VASH1 (C terminal). The exact sequence is proprietary. NP_055724
Database link: Q7L8A9
- Human brain tissue lysate and mouse brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab176114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 41 kDa.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|IHC-P||Use a concentration of 5 µg/ml.|
FunctionAngiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity.
Tissue specificityPreferentially expressed in endothelial cells. Highly expressed in fetal organs. Expressed in brain and placenta, and at lower level in heart and kidney. Highly detected in microvessels endothelial cells of atherosclerotic lesions.
Sequence similaritiesBelongs to the vasohibin family.
modifications2 major forms (42 and 36 kDa) and 2 minors (32 and 27 kDa) may be processed by proteolytic cleavage. The largest form (42 kDa) seems to be secreted and the other major form (63 kDa) seems to accumulate within the cells or pericellular milieu. Polypeptide consisting of Met-77 to Arg-318 may correspond to the 27 kDa form and that consisting of Met-77 to Val-365 may correspond to the 36 kDa form.
- Information by UniProt
- KIAA1036 antibody
- VASH antibody
- VASH1 antibody
Immunohistochemical analysis of mouse brain tissue labeling VASH1 with ab176114 at 5 μg/ml dilution.
All lanes : Anti-VASH1 antibody - C-terminal (ab176114) at 1 µg/ml
Lane 1 : Human brain tissue lysate with without blocking peptide
Lane 2 : Human brain tissue lysate with blocking peptide
Lysates/proteins at 15 µg per lane.
Developed using the ECL technique.
Predicted band size: 41 kDa
This product has been referenced in:
- Tang XW & Qin QX miR-335-5p induces insulin resistance and pancreatic islet ß-cell secretion in gestational diabetes mellitus mice through VASH1-mediated TGF-ß signaling pathway. J Cell Physiol 234:6654-6666 (2019). Read more (PubMed: 30341900) »
- Zhou CF et al. Cervical squamous cell carcinoma-secreted exosomal miR-221-3p promotes lymphangiogenesis and lymphatic metastasis by targeting VASH1. Oncogene 38:1256-1268 (2019). Read more (PubMed: 30254211) »