Overview

  • Product name

    Anti-VASH1 antibody [EPR17420]
    See all VASH1 primary antibodies
  • Description

    Rabbit monoclonal [EPR17420] to VASH1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human VASH1 aa 250-350. The exact sequence is proprietary.
    Database link: Q7L8A9

  • Positive control

    • WB: HeLa, HUVEC, A431, RAW 264.7, PC-12 and NIH/3T3 whole cell lysates; Mouse brain, heart and kidney lysates; Rat brain and heart lysates; Human fetal kidney and fetal spleen lysates. ICC/IF: HUVEC cells. IP: A431 whole cell lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR17420
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab199732 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
ICC/IF 1/250.
IP 1/30.

Target

  • Function

    Angiogenesis inhibitor. Inhibits migration, proliferation and network formation by endothelial cells as well as angiogenesis. This inhibitory effect is selective to endothelial cells as it does not affect the migration of smooth muscle cells or fibroblasts. Does not affect the proliferation of cancer cells in vitro, but inhibits tumor growth and tumor angiogenesis. Acts in an autocrine manner. Inhibits artery neointimal formation and macrophage infiltration. Exhibits heparin-binding activity.
  • Tissue specificity

    Preferentially expressed in endothelial cells. Highly expressed in fetal organs. Expressed in brain and placenta, and at lower level in heart and kidney. Highly detected in microvessels endothelial cells of atherosclerotic lesions.
  • Sequence similarities

    Belongs to the vasohibin family.
  • Post-translational
    modifications

    2 major forms (42 and 36 kDa) and 2 minors (32 and 27 kDa) may be processed by proteolytic cleavage. The largest form (42 kDa) seems to be secreted and the other major form (63 kDa) seems to accumulate within the cells or pericellular milieu. Polypeptide consisting of Met-77 to Arg-318 may correspond to the 27 kDa form and that consisting of Met-77 to Val-365 may correspond to the 36 kDa form.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • KIAA1036 antibody
    • VASH antibody
    • VASH1 antibody
    • VASH1_HUMAN antibody
    • Vasohibin 1 antibody
    • Vasohibin-1 antibody
    see all

Images

  • All lanes : Anti-VASH1 antibody [EPR17420] (ab199732) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HUVEC (Human umbilical vein endothelial cell line) whole cell lysate
    Lane 3 : A431 (Human epidermoid carcinoma) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-VASH1 antibody [EPR17420] (ab199732) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Rat brain lysate
    Lane 5 : Rat heart lysate
    Lane 6 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
    Lane 7 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate
    Lane 8 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-VASH1 antibody [EPR17420] (ab199732) at 1/1000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 41 kDa
    Observed band size: 41 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • VASH1 was immunoprecipitated from 1mg of A431 (Human epidermoid carcinoma) whole cell lysate with ab199732 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199732 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

    Lane 1: A431 whole cell lysate 10 µg (Input). Lane 2: ab199732 IP in A431 whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199732 in A431 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

References

This product has been referenced in:

  • Huang W  et al. Vasohibin 1 inhibits Adriamycin resistance in osteosarcoma cells via the protein kinase B signaling pathway. Oncol Lett 15:5983-5988 (2018). Read more (PubMed: 29556314) »
  • Zhang T  et al. MicroRNA-4530 promotes angiogenesis by targeting VASH1 in breast carcinoma cells. Oncol Lett 14:111-118 (2017). Read more (PubMed: 28693142) »
See all 2 Publications for this product

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