Key features and details
- Rabbit polyclonal to VASH2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-VASH2 antibody
DescriptionRabbit polyclonal to VASH2
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Recombinant full length protein corresponding to Human VASH2 aa 1-355.
MTGSAADTHRCPHPKGAKGTRSRSSHARPVSLATSGGSEEEDKDGGVLFH VNKSGFPIDSHTWERMWMHVAKVHPKGGEMVGAIRNAAFLAKPSIPQVPN YRLSMTIPDWLQAIQNYMKTLQYNHTGTQFFEIRKMRPLSGLMETAKEMT RESLPIKCLEAVILGIYLTNGQPSIERFPISFKTYFSGNYFHHVVLGIYC NGRYGSLGMSRRAELMDKPLTFRTLSDLIFDFEDSYKKYLHTVKKVKIGL YVPHEPHSFQPIEWKQLVLNVSKMLRADIRKELEKYARDMRMKILKPASA HSPTQVRSRGKSLSPRRRQASPPRRLGRREKSPALPEKKVADLSTLNEVG YQIRI
Database link: Q86V25
- WB: Mouse liver and kidney lysates. IHC-P: Human colon cancer and gastric cancer tissues. ICC/IF: A549 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab224723 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/5000. Detects a band of approximately 41 kDa (predicted molecular weight: 40 kDa).|
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
FunctionAngiogenesis inhibitor. Inhibits network formation by endothelial cells.
Sequence similaritiesBelongs to the vasohibin family.
Developmental stageExpressed in various embryonic organs at 6 to 12 embryonic weeks. Detected in vessels from 20-week embryonic organs as well as in endothelial cells from large vessels in neonate.
- Information by UniProt
- FLJ12505 antibody
- OTTHUMP00000035013 antibody
- OTTHUMP00000035017 antibody
All lanes : Anti-VASH2 antibody (ab224723) at 1/500 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse kidney lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 40 kDa
Observed band size: 41 kDa why is the actual band size different from the predicted?
Paraffin-embedded human colon cancer tissue stained for VASH2 using ab224723 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human gastric cancer tissue stained for VASH2 using ab224723 at 1/100 dilution in immunohistochemical analysis.
A549 (human lung carcinoma cell line) cells stained for VASH2 (green) using ab224723 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab224723 has not yet been referenced specifically in any publications.