The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration.
1/500 - 1/5000.
Vasopressin, also known as arginine vasopressin (AVP) or antidiuretic hormone (ADH), is a posterior pituitary hormone that is synthesised in the hypothalamus. Vasopressin is synthesised as a precursor protein that consists of arginine vasopressin and two associated proteins, neurophysin 2 and the glycopeptide copeptin. Vasopressin, together with its carrier protein neurophysin II, is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney and also causes vasoconstriction of the peripheral vessels. Vasopressin can also contract smooth muscle during parturition and lactation. It also plays a role in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in the vasopressin precursor cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI), which is characterised by persistant thirst, polydipsia and polyuria.
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Vasopressin antibody (ab39363)Image from Ettrup KS et al, J Chem Neuroanat. 2010 May;39(3):151-65. Epub 2009 Dec 28, Fig 2, doi:10.1016/j.jchemneu.2009.12.004.
Minipigs were deeply anesthetized with a combination of midazolam and
ketamine, prior to transcardial perfusion with phosphate buffered 4%
paraformaldehyde (pH 7.4). After perfusion, the brains were removed with special care taken to preserve the optic chiasm and the median eminence. Blocks of tissue containing the hypothalami
were dissected, postfixed in the same fixative for 1 day and subsequently cryoprotected in 30% sucrose for 3–4 days, prior to freezing. 10 series of 40-mm thick
coronal (6 animals), sagittal (1 animal), and horizontal (1 animal) cryostat
sections were collected. Coronal sections for immunohistochemistry were maintained at -18°C as free-floating sections in a cryoprotectant poly-ethylene glycol solution for up to four weeks.
Immunohistochemistry was performed using the avidin-biotin method. Accordingly,
free-floating sections were
first rinsed in Tris-buffered saline (TBS; 0.05 M; pH 7.4) for 15 minutes. Incubations with
avidin (0.1%) and