Anti-Vasopressin antibody (ab64542)


  • Product name
  • Description
    Rabbit polyclonal to Vasopressin
  • Host species
  • Tested applications
    Suitable for: IHC-P, ELISAmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Human Vasopressin.

    (Peptide available as ab68667.)

  • Positive control
    • This antibody gave a positive signal in IHC when used to probe human hypothalamus tissue.



Our Abpromise guarantee covers the use of ab64542 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      Vasopressin, also known as arginine vasopressin (AVP) or antidiuretic hormone (ADH), is a posterior pituitary hormone that is synthesised in the hypothalamus. Vasopressin is synthesised as a precursor protein that consists of arginine vasopressin and two associated proteins, neurophysin 2 and the glycopeptide copeptin. Vasopressin, together with its carrier protein neurophysin II, is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney and also causes vasoconstriction of the peripheral vessels. Vasopressin can also contract smooth muscle during parturition and lactation. It also plays a role in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in the vasopressin precursor cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI), which is characterised by persistant thirst, polydipsia and polyuria.
    • Cellular localization
    • Database links
    • Alternative names
      • ADH antibody
      • Antidiuretic hormone antibody
      • Arginine vasopressin neurophysin II antibody
      • ARVP antibody
      • AVP antibody
      • AVP NPII antibody
      • copeptin antibody
      • Vasopressin neurophysin II copeptin antibody
      • VP antibody
      see all


    • IHC image of Vasopressin staining in human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab64542, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
    • ELISA using ab64542 at varying antibody concentrations. The black line indicates binding to the vasopressin peptide ab68667


    ab64542 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Thank you for your patience.

    Please accept my apologies for the delay of your case. I was in contact with our lab, and it looks like we made a datasheet mistake by giving such a low minimum concentration of the antibody for the use in ELISA....

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    Thank you for your call.

    I am happy to confirm that ab64542 is tested and guaranteed for ELISA and that ab68667 (Vasopressin peptide) is a suitable positive control for ELISA.

    ab68667 is a short peptide and it can sometimes be trick...

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    Thank you for contacting us and for your patience.

    We were in contact with our lab, hence the delay.

    Here (in a short version) is our protocol:

    "The ELISA performed is only against the Immunising peptide in an ...

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    Thank you for your enquiry.

    Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

    In this instance, none of our Vaso...

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    Thank you for contacting us. The MSDS can be downloaded online form our website. The link is given on every antibody datasheet in storage buffer section. Please also find attached with this email. Ab3856 does not contain any prese...

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