Overview

  • Product name

  • Description

    Rabbit polyclonal to Vasopressin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-FoFr, IHC-P, ELISAmore details
    Unsuitable for: WB
  • Species reactivity

    Reacts with: Sheep, Human
    Predicted to work with: Mouse, Rat, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide corresponding to Human Vasopressin aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab68668)

  • Positive control

    • This antibody gave a positive signal in IHC when used to probe human hypothalamus tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab68669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/100.
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use a concentration of 0.002 µg/ml.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance

      Vasopressin, also known as arginine vasopressin (AVP) or antidiuretic hormone (ADH), is a posterior pituitary hormone that is synthesised in the hypothalamus. Vasopressin is synthesised as a precursor protein that consists of arginine vasopressin and two associated proteins, neurophysin 2 and the glycopeptide copeptin. Vasopressin, together with its carrier protein neurophysin II, is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney and also causes vasoconstriction of the peripheral vessels. Vasopressin can also contract smooth muscle during parturition and lactation. It also plays a role in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in the vasopressin precursor cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI), which is characterised by persistant thirst, polydipsia and polyuria.
    • Cellular localization

      Secreted
    • Database links

    • Alternative names

      • ADH antibody
      • Antidiuretic hormone antibody
      • Arginine vasopressin neurophysin II antibody
      • ARVP antibody
      • AVP antibody
      • AVP NPII antibody
      • copeptin antibody
      • Vasopressin neurophysin II copeptin antibody
      • VP antibody
      see all

    Images

    • ab68669 staining Vasopressin in sheep brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100, blocked with 5% serum for 1 hour at 23°C and antigen retrieval was by heat mediation in 0.1M citrate buffer pH 6.0. The sample was incubated with primary antibody (1/100 in 0.3% Triton X-100) at 4°C for 12 hours. A Biotin-conjugated anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

      See Abreview

    • IHC image of Vasopressin staining in human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68669 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
    • ELISA using ab68669 at varying antibody concentrations. The black line indicates binding to the vasopressin peptide ab68668.

    References

    This product has been referenced in:

    • Zhou ZB  et al. Sevoflurane-induced down-regulation of hippocampal oxytocin and arginine vasopressin impairs juvenile social behavioral abilities. J Mol Neurosci 56:70-7 (2015). IHC . Read more (PubMed: 25417719) »
    See 1 Publication for this product

    Customer reviews and Q&As

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    1-3 of 3 Abreviews

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Hypothalamus)
    Permeabilization
    Yes - 0.5% Triton X-100
    Specification
    Hypothalamus
    Blocking step
    Serum as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 4°C
    Fixative
    Paraformaldehyde

    Kevin Zhang

    Verified customer

    Submitted Sep 06 2017

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.1M Citrate Buffer pH 6.0
    Permeabilization
    Yes - 0.3% Triton X-100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Fixative
    Paraformaldehyde

    Miss. Juliana Garcia da Silva Fonseca

    Verified customer

    Submitted Aug 13 2014

    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 0.1M Citrate Buffer pH 6.0
    Sample
    Sheep Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    Yes - 0.3% Triton X-100
    Fixative
    Paraformaldehyde

    Miss. Juliana Garcia da Silva Fonseca

    Verified customer

    Submitted Aug 13 2014

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