Anti-Vasopressin antibody (ab68669)

Reviews (3)Q&A (1)References (1)

Overview

  • Product name

    Anti-Vasopressin antibody
    See all Vasopressin primary antibodies

  • Description

    Rabbit polyclonal to Vasopressin

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-FoFr, IHC-P, ELISAmore details
    Unsuitable for: WB

  • Species reactivity

    Reacts with: Sheep, Human
    Predicted to work with: Mouse, Rat, Cow, Cat, Dog, Pig

  • Immunogen

    Synthetic peptide corresponding to Human Vasopressin aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab68668)

  • Positive control

    • This antibody gave a positive signal in IHC when used to probe human hypothalamus tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab68669 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/100.
IHC-P Use a concentration of 1 - 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ELISA Use a concentration of 0.002 µg/ml.
  • Application notes
    Is unsuitable for WB.

    • Target

    • Relevance

      Vasopressin, also known as arginine vasopressin (AVP) or antidiuretic hormone (ADH), is a posterior pituitary hormone that is synthesised in the hypothalamus. Vasopressin is synthesised as a precursor protein that consists of arginine vasopressin and two associated proteins, neurophysin 2 and the glycopeptide copeptin. Vasopressin, together with its carrier protein neurophysin II, is packaged into neurosecretory vesicles and transported axonally to the nerve endings in the neurohypophysis, where it is either stored or secreted into the bloodstream. Vasopressin acts as a growth factor by enhancing pH regulation through acid-base transport systems. It has a direct antidiuretic action on the kidney and also causes vasoconstriction of the peripheral vessels. Vasopressin can also contract smooth muscle during parturition and lactation. It also plays a role in cognition, tolerance, adaptation and complex sexual and maternal behaviour, as well as in the regulation of water excretion and cardiovascular functions. Mutations in the vasopressin precursor cause autosomal dominant neurohypophyseal diabetes insipidus (ADNDI), which is characterised by persistant thirst, polydipsia and polyuria.

    • Cellular localization

      Secreted

    • Database links

      see all

    • Alternative names

      • ADH antibody
      • Antidiuretic hormone antibody
      • Arginine vasopressin neurophysin II antibody
      • ARVP antibody
      • AVP antibody
      • AVP NPII antibody
      • copeptin antibody
      • Vasopressin neurophysin II copeptin antibody
      • VP antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody (ab68669)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody (ab68669)This image is courtesy of an Abreview submitted by Juliana Garcia da Silva Fonseca

      ab68669 staining Vasopressin in sheep brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde, permeablized with 0.3% Triton X-100, blocked with 5% serum for 1 hour at 23°C and antigen retrieval was by heat mediation in 0.1M citrate buffer pH 6.0. The sample was incubated with primary antibody (1/100 in 0.3% Triton X-100) at 4°C for 12 hours. A Biotin-conjugated anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody (ab68669)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vasopressin antibody (ab68669)
      IHC image of Vasopressin staining in human Hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68669 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
    • ELISA - Anti-Vasopressin antibody (ab68669)
      ELISA - Anti-Vasopressin antibody (ab68669)
      ELISA using ab68669 at varying antibody concentrations. The black line indicates binding to the vasopressin peptide ab68668.

    References

    This product has been referenced in:

    • Zhou ZB  et al. Sevoflurane-induced down-regulation of hippocampal oxytocin and arginine vasopressin impairs juvenile social behavioral abilities. J Mol Neurosci 56:70-7 (2015). IHC . Read more (PubMed: 25417719) »
    See 1 Publication for this product

    Publishing research using ab68669? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

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    Submit a question

    Question

    I am interested in ordering Vasopressin antibody from you, which will be used for coupling to dynabeads magnetic beads  for IP of Vasopressin from serum sample. But I found four types (ab39363  , ab64542 ,ab68669  ,ab48322  ) from your website. Could you tell me which one to choose? Thank you.

    Read More
    Answer

    Thank you for your enquiry.

    Although we produce many in-house antibodies, we obtain other antibodies from a wide range of sources. Therefore, comparison experiments will not often have been done.

    In this instance, none of our Vasopressin antibodies have been tested in IP.Therefore, I can offer a discount off a future purchase if you buy an anti-Vasopressin antibody now, test it in IP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free PRIMARY ANTIBODY.

    If you wish to be more certain about which product to chose, I can suggest choosing the antibody that has been best characterized. By this I mean the antibody that has been tested in the most applications and has the most data available (on the datasheet).


    If you are interested in our Abreview offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed a product in IP. I will then send a discount code. This code must be issued before purchase so please wait for my reply before ordering.

    2. Purchase either by phone, fax, or online (www.abcam.com).

    3. Test it in IP.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any PRIMARY ANTIBODY ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for IP, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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