Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-Vasopressin antibody

Good
Abreviews
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.1M Citrate Buffer pH 6.0
Sample
Sheep Tissue sections (Brain)
Specification
Brain
Permeabilization
Yes - 0.3% Triton X-100
Fixative
Paraformaldehyde

Other product details

Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 0.3% Triton X-100
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Anti-rabbit IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/400

Miss. Juliana Garcia da Silva Fonseca

Verified customer

Submitted Aug 13 2014

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